RT Journal Article
T1 A comparative study of the preventative effects exerted by two probiotics, Lactobacillus reuteri and Lactobacillus fermentum, in the trinitrobenzenesulfonic  acid model of rat colitis
A1 Peran, Laura
A1 Sierra, Saleta
A1 Comalada, Mònica
A1 Lara-Villoslada, Federico
A1 Bailón, Elvira
A1 Nieto, Ana
A1 Concha, Angel
A1 Olivares, Mónica
A1 Zarzuelo, Antonio
A1 Xaus, Jordi
A1 Gálvez, Julio
K1 Probiotics
K1 Inflammatory bowel diseases
K1 Immunomodulation
K1 Anti-inflammatory activity
K1 Colitis
K1 Colon
K1 Diarrea
K1 Ácidos Grasos Volátiles
K1 Heces
K1 Femenino
K1 Contenido Digestivo
K1 Glutatión
K1 Concentración de Iones de Hidrógeno
K1 Enfermedades Inflamatorias del Intestino
K1 Mucosa Intestinal
K1 Lactobacillus fermentum
K1 Lactobacillus reuteri
K1 Modelos Animales
K1 Músculo Esquelético
K1 Tamaño de los Órganos
K1 Peroxidasa
K1 Probióticos
K1 Ratas
K1 Ratas Wistar
K1 Bazo
K1 Timo
K1 Ácido Trinitrobencenosulfónico
K1 Factor de Necrosis Tumoral alfa
K1 Animales
AB The intestinal anti-inflammatory effects of two probiotics isolated from breast milk, Lactobacillus reuteri and L. fermentum, were evaluated and compared in the trinitrobenzenesulfonic acid (TNBS) model of rat colitis. Colitis was induced in rats by intracolonic administration of 10 mg TNBS dissolved in 50% ethanol (0.25 ml). Either L. reuteri or L. fermentum was daily administered orally (5 x 10(8) colony-forming units suspended in 0.5 ml skimmed milk) to each group of rats (n 10) for 3 weeks, starting 2 weeks before colitis induction. Colonic damage was evaluated histologically and biochemically, and the colonic luminal contents were used for bacterial studies and for SCFA production. Both probiotics showed intestinal anti-inflammatory effects in this model of experimental colitis, as evidenced histologically and by a significant reduction of colonic myeloperoxidase activity (P<0.05). L. fermentum significantly counteracted the colonic glutathione depletion induced by the inflammatory process. In addition, both probiotics lowered colonic TNFalpha levels (P<0.01) and inducible NO synthase expression when compared with non-treated rats; however, the decrease in colonic cyclo-oxygenase-2 expression was only achieved with L.fermentum administration. Finally, the two probiotics induced the growth of Lactobacilli species in comparison with control colitic rats, but the production of SCFA in colonic contents was only increased when L. fermentum was given. In conclusion, L. fermentum can exert beneficial immunomodulatory properties in inflammatory bowel disease, being more effective than L. reuteri, a probiotic with reputed efficacy in promoting beneficial effects on human health.
PB Cambridge University Press
SN 0007-1145
YR 2007
FD 2007-01-12
LK http://hdl.handle.net/10668/646
UL http://hdl.handle.net/10668/646
LA en
NO Peran L, Sierra S, Comalada M, Lara-Villoslada F, Bailón E, Nieto A, et al. A comparative study of the preventative effects exerted by two probiotics, Lactobacillus reuteri and Lactobacillus fermentum, in the trinitrobenzenesulfonic acid model of rat colitis. Br. J. Nutr.. 2007                         ; 97(1):96-103
NO Comparative Study; Journal Article; Research Support, Non-U.S. Gov't;
DS RISalud
RD Apr 7, 2025