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Title: Retrieval of germinal zone neural stem cells from the cerebrospinal fluid of premature infants with intraventricular hemorrhage
Authors: Fernández-Muñoz, Beatriz
Rosell-Valle, Cristina
Ferrari, Daniela
Alba-Amador, Julia
Montiel, Miguel Ángel
Campos-Cuerva, Rafael
Lopez-Navas, Luis
Muñoz-Escalona, María
Martín-López, María
Profico, Daniela Celeste
Blanco, Manuel Francisco
Giorgetti, Alessandra
González-Muñoz, Elena
Márquez-Rivas, Javier
Sanchez-Pernaute, Rosario
metadata.dc.contributor.authoraffiliation: [Fernández-Muñoz,B; Rosell-Valle,C; Alba-Amador,J; Montiel,MÁ; Campos-Cuerva,R; Muñoz-Escalona,M; Martín-López,M; Blanco,MF] Unidad de Producción y Reprogramación Celular (UPRC), Red Andaluza para el diseño y traslación de Terapias Avanzadas, Sevilla, Spain. [Fernández-Muñoz,B; Martín-López,M; Márquez-Rivas,J] Grupo de Neurociencia aplicada, Instituto de Biomedicina de Sevilla, Sevilla, Spain. [Ferrari,D] Department of Biotechnology and Biosciences, University Milan-Bicocca, Milan, Italy. [Campos-Cuerva,R] Centro de Transfusiones, Tejidos y Células de Sevilla (CTTS), Sevilla, Spain. [Lopez-Navas,L; Sanchez-Pernaute,R] Departamento de Preclínica, Red Andaluza de Diseño y Traslación de Terapias Avanzadas, Sevilla, Spain. [Profico,DC] Fondazione IRCCS Casa Sollievo della Sofferenza, Production Unit of Advanced Therapies (UPTA), San Giovanni Rotondo, Italy. [Giorgetti,A] Regenerative Medicine Program, Bellvitge Biomedical Research Institute (IDIBELL); Program for Translation of Regenerative Medicine in Catalonia (P-CMRC), Barcelona, Spain. [González-Muñoz,E] Department of Cell Biology, Genetics and Physiology, University of Málaga, Málaga, Spain. [González-Muñoz,E] Department of Regenerative Nanomedicine, Andalusian Center for Nanomedicine and Biotechnology-BIONAND, Málaga, Spain. [González-Muñoz,E] Networking Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN). Carlos III Health Institute (ISCIII), Spain. [Márquez-Rivas,J] Neurosurgery Department, Hospital Virgen del Rocío, Sevilla, Spain.
Keywords: Cerebrospinal fluid;Germinal zone;Intraventricular hemorrhage;Neural stem cell;Neurogenesis;Premature infant;Líquido cefalorraquídeo;Centro germinal;Hemorragia cerebral intraventricular;Células madre nerviosas;Recién nacido prematuro
metadata.dc.subject.mesh: Medical Subject Headings::Organisms::Eukaryota::Animals
Medical Subject Headings::Diseases::Nervous System Diseases::Central Nervous System Diseases::Brain Diseases::Cerebrovascular Disorders::Intracranial Hemorrhages::Cerebral Hemorrhage
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Diagnosis::Diagnostic Techniques and Procedures::Diagnostic Techniques, Surgical::Endoscopy
Medical Subject Headings::Check Tags::Female
Medical Subject Headings::Phenomena and Processes::Genetic Phenomena::Genetic Processes::Gene Expression Regulation::Gene Expression Regulation, Developmental
Medical Subject Headings::Persons::Persons::Age Groups::Infant::Infant, Newborn::Infant, Premature
Medical Subject Headings::Check Tags::Male
Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Rodentia::Muridae::Murinae::Mice::Mice, Mutant Strains::Mice, Nude
Medical Subject Headings::Anatomy::Cells::Stem Cells::Neural Stem Cells
Medical Subject Headings::Anatomy::Fluids and Secretions::Body Fluids::Extracellular Fluid::Cerebrospinal Fluid
Issue Date: 2020
Publisher: Wiley Periodicals, Inc. on behalf of AlphaMed Press
Citation: Fernández-Muñoz B, Rosell-Valle C, Ferrari D, Alba-Amador J, Montiel MÁ, Campos-Cuerva R, et al. Retrieval of germinal zone neural stem cells from the cerebrospinal fluid of premature infants with intraventricular hemorrhage. Stem Cells Transl Med. 2020 Sep;9(9):1085-1101
Abstract: Intraventricular hemorrhage is a common cause of morbidity and mortality in premature infants. The rupture of the germinal zone into the ventricles entails loss of neural stem cells and disturbs the normal cytoarchitecture of the region, compromising late neurogliogenesis. Here we demonstrate that neural stem cells can be easily and robustly isolated from the hemorrhagic cerebrospinal fluid obtained during therapeutic neuroendoscopic lavage in preterm infants with severe intraventricular hemorrhage. Our analyses demonstrate that these neural stem cells, although similar to human fetal cell lines, display distinctive hallmarks related to their regional and developmental origin in the germinal zone of the ventral forebrain, the ganglionic eminences that give rise to interneurons and oligodendrocytes. These cells can be expanded, cryopreserved, and differentiated in vitro and in vivo in the brain of nude mice and show no sign of tumoral transformation 6 months after transplantation. This novel class of neural stem cells poses no ethical concerns, as the fluid is usually discarded, and could be useful for the development of an autologous therapy for preterm infants, aiming to restore late neurogliogenesis and attenuate neurocognitive deficits. Furthermore, these cells represent a valuable tool for the study of the final stages of human brain development and germinal zone biology.
metadata.dc.identifier.doi: 10.1002/sctm.19-0323
ISSN: 2157-6564 (Print)
2157-6580 (Online)
Appears in Collections:01- Artículos - Biobanco del Sistema Sanitario Público de Andalucía
01- Artículos - BIONAND - Centro Andaluz de Nanomedicina y Biotecnología
01- Artículos - Hospital Virgen del Rocío
01- Artículos - IBIS. Instituto de Biomedicina de Sevilla
01- Artículos - Red Andaluza de Diseño y Traslación de Terapias Avanzadas

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