RT Journal Article
T1 Retrieval of germinal zone neural stem cells from the cerebrospinal fluid of premature infants with intraventricular hemorrhage
A1 Fernández-Muñoz, Beatriz
A1 Rosell-Valle, Cristina
A1 Ferrari, Daniela
A1 Alba-Amador, Julia
A1 Montiel, Miguel Ángel
A1 Campos-Cuerva, Rafael
A1 Lopez-Navas, Luis
A1 Muñoz-Escalona, María
A1 Martín-López, María
A1 Profico, Daniela Celeste
A1 Blanco, Manuel Francisco
A1 Giorgetti, Alessandra
A1 González-Muñoz, Elena
A1 Márquez-Rivas, Javier
A1 Sanchez-Pernaute, Rosario
K1 Cerebrospinal fluid
K1 Germinal zone
K1 Intraventricular hemorrhage
K1 Neural stem cell
K1 Neurogenesis
K1 Premature infant
K1 Líquido cefalorraquídeo
K1 Centro germinal
K1 Hemorragia cerebral intraventricular
K1 Células madre nerviosas
K1 Recién nacido prematuro
AB Intraventricular hemorrhage is a common cause of morbidity and mortality in premature infants. The rupture of the germinal zone into the ventricles entails loss of neural stem cells and disturbs the normal cytoarchitecture of the region, compromising late neurogliogenesis. Here we demonstrate that neural stem cells can be easily and robustly isolated from the hemorrhagic cerebrospinal fluid obtained during therapeutic neuroendoscopic lavage in preterm infants with severe intraventricular hemorrhage. Our analyses demonstrate that these neural stem cells, although similar to human fetal cell lines, display distinctive hallmarks related to their regional and developmental origin in the germinal zone of the ventral forebrain, the ganglionic eminences that give rise to interneurons and oligodendrocytes. These cells can be expanded, cryopreserved, and differentiated in vitro and in vivo in the brain of nude mice and show no sign of tumoral transformation 6 months after transplantation. This novel class of neural stem cells poses no ethical concerns, as the fluid is usually discarded, and could be useful for the development of an autologous therapy for preterm infants, aiming to restore late neurogliogenesis and attenuate neurocognitive deficits. Furthermore, these cells represent a valuable tool for the study of the final stages of human brain development and germinal zone biology.
PB Wiley Periodicals, Inc. on behalf of AlphaMed Press
SN 2157-6564
YR 2020
FD 2020
LK http://hdl.handle.net/10668/3868
UL http://hdl.handle.net/10668/3868
LA en
NO Fernández-Muñoz B, Rosell-Valle C, Ferrari D, Alba-Amador J, Montiel MÁ, Campos-Cuerva R, et al. Retrieval of germinal zone neural stem cells from the cerebrospinal fluid of premature infants with intraventricular hemorrhage. Stem Cells Transl Med. 2020 Sep;9(9):1085-1101
DS RISalud
RD Apr 20, 2025