Publication: Metabolomic Analysis of The Chemical Diversity of South Africa Leaf Litter Fungal Species Using an Epigenetic Culture-Based Approach.
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Date
2021-07-14
Authors
Serrano, Rachel
González-Menéndez, Víctor
Martínez, Germán
Toro, Clara
Martín, Jesús
Genilloud, Olga
Tormo, José R
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Abstract
Microbial natural products are an invaluable resource for the biotechnological industry. Genome mining studies have highlighted the huge biosynthetic potential of fungi, which is underexploited by standard fermentation conditions. Epigenetic effectors and/or cultivation-based approaches have successfully been applied to activate cryptic biosynthetic pathways in order to produce the chemical diversity suggested in available fungal genomes. The addition of Suberoylanilide Hydroxamic Acid to fermentation processes was evaluated to assess its effect on the metabolomic diversity of a taxonomically diverse fungal population. Here, metabolomic methodologies were implemented to identify changes in secondary metabolite profiles to determine the best fermentation conditions. The results confirmed previously described effects of the epigenetic modifier on the metabolism of a population of 232 wide diverse South Africa fungal strains cultured in different fermentation media where the induction of differential metabolites was observed. Furthermore, one solid-state fermentation (BRFT medium), two classic successful liquid fermentation media (LSFM and YES) and two new liquid media formulations (MCKX and SMK-II) were compared to identify the most productive conditions for the different populations of taxonomic subgroups.
Description
MeSH Terms
Biological Products
Biosynthetic Pathways
Biotechnology
Culture Media
Epigenesis, Genetic
Fermentation
Fungi
Genome, Fungal
Metabolomics
Plant Leaves
South Africa
Biosynthetic Pathways
Biotechnology
Culture Media
Epigenesis, Genetic
Fermentation
Fungi
Genome, Fungal
Metabolomics
Plant Leaves
South Africa
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CIE Terms
Keywords
chemical diversity, epigenetic modifier, fungal fermentations, metabolomic