RT Journal Article
T1 Metabolomic Analysis of The Chemical Diversity of South Africa Leaf Litter Fungal Species Using an Epigenetic Culture-Based Approach.
A1 Serrano, Rachel
A1 González-Menéndez, Víctor
A1 Martínez, Germán
A1 Toro, Clara
A1 Martín, Jesús
A1 Genilloud, Olga
A1 Tormo, José R
K1 chemical diversity
K1 epigenetic modifier
K1 fungal fermentations
K1 metabolomic
AB Microbial natural products are an invaluable resource for the biotechnological industry. Genome mining studies have highlighted the huge biosynthetic potential of fungi, which is underexploited by standard fermentation conditions. Epigenetic effectors and/or cultivation-based approaches have successfully been applied to activate cryptic biosynthetic pathways in order to produce the chemical diversity suggested in available fungal genomes. The addition of Suberoylanilide Hydroxamic Acid to fermentation processes was evaluated to assess its effect on the metabolomic diversity of a taxonomically diverse fungal population. Here, metabolomic methodologies were implemented to identify changes in secondary metabolite profiles to determine the best fermentation conditions. The results confirmed previously described effects of the epigenetic modifier on the metabolism of a population of 232 wide diverse South Africa fungal strains cultured in different fermentation media where the induction of differential metabolites was observed. Furthermore, one solid-state fermentation (BRFT medium), two classic successful liquid fermentation media (LSFM and YES) and two new liquid media formulations (MCKX and SMK-II) were compared to identify the most productive conditions for the different populations of taxonomic subgroups.
YR 2021
FD 2021-07-14
LK http://hdl.handle.net/10668/18244
UL http://hdl.handle.net/10668/18244
LA en
DS RISalud
RD Apr 7, 2025