Publication: Peripheral blood mononuclear cells-expressed miRNA profiles derived from children with metabolic-associated fatty liver disease and insulin resistance.
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Date
2022-08-31
Authors
Oses, Maddi
Medrano, María
Margareto Sanchez, Javier
Portillo, Maria P
Aguilera, Concepcion Maria
Altmäe, Signe
Labayen, Idoia
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Abstract
miRNA have been proposed as potential biomarkers of metabolic diseases. To identify potential miRNA biomarkers of early metabolic-associated fatty liver disease (MAFLD) and/or insulin resistance (IR) in preadolescent children. A total of 70 preadolescents, aged 8.5-12 years old participated in the study. Hepatic fat was assessed by magnetic resonance imaging. Fasting blood biochemical parameters were measured and HOMA-IR calculated. Peripheral blood mononuclear cells (PBMC)-derived miRNA profiles associated with MAFLD (≥5.5% hepatic fat) and IR (HOMA-IR ≥2.5) were identified using untargeted high-throughput miRNAs sequencing (RNA-seq). A total of 2123 PBMC-derived miRNAs were identified in children with (21.4%) or without MAFLD. Among them, hsa-miR-143-3p, hsa-miR-142-5p and hsa-miR-660-5p were up-regulated, and p-hsa-miR-247, hsa-let-7a-5p and hsa-miR-6823-3p down-regulated. Importantly, children with MAFLD had consistently higher miR-660-5p expression levels than their peers without it (p Our study results suggest circulating miR-660-5p as a potential biomarker of the presence of MAFLD in preadolescent children while circulating miR-320a, miR-142-3p, miR-190a-5p, miR-374a-5p and let-7 family miRNAs could serve as potential biomarkers of IR in children.
Description
MeSH Terms
Child
Humans
Leukocytes, Mononuclear
Insulin Resistance
MicroRNAs
Biomarkers
Liver Diseases
Humans
Leukocytes, Mononuclear
Insulin Resistance
MicroRNAs
Biomarkers
Liver Diseases
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CIE Terms
Keywords
MiRNA, children, fatty liver, insulin resistance, metabolic diseases, obesity