RT Journal Article
T1 Peripheral blood mononuclear cells-expressed miRNA profiles derived from children with metabolic-associated fatty liver disease and insulin resistance.
A1 Oses, Maddi
A1 Medrano, María
A1 Margareto Sanchez, Javier
A1 Portillo, Maria P
A1 Aguilera, Concepcion Maria
A1 Altmäe, Signe
A1 Labayen, Idoia
K1 MiRNA
K1 children
K1 fatty liver
K1 insulin resistance
K1 metabolic diseases
K1 obesity
AB miRNA have been proposed as potential biomarkers of metabolic diseases. To identify potential miRNA biomarkers of early metabolic-associated fatty liver disease (MAFLD) and/or insulin resistance (IR) in preadolescent children. A total of 70 preadolescents, aged 8.5-12 years old participated in the study. Hepatic fat was assessed by magnetic resonance imaging. Fasting blood biochemical parameters were measured and HOMA-IR calculated. Peripheral blood mononuclear cells (PBMC)-derived miRNA profiles associated with MAFLD (≥5.5% hepatic fat) and IR (HOMA-IR ≥2.5) were identified using untargeted high-throughput miRNAs sequencing (RNA-seq). A total of 2123 PBMC-derived miRNAs were identified in children with (21.4%) or without MAFLD. Among them, hsa-miR-143-3p, hsa-miR-142-5p and hsa-miR-660-5p were up-regulated, and p-hsa-miR-247, hsa-let-7a-5p and hsa-miR-6823-3p down-regulated. Importantly, children with MAFLD had consistently higher miR-660-5p expression levels than their peers without it (p  Our study results suggest circulating miR-660-5p as a potential biomarker of the presence of MAFLD in preadolescent children while circulating miR-320a, miR-142-3p, miR-190a-5p, miR-374a-5p and let-7 family miRNAs could serve as potential biomarkers of IR in children.
YR 2022
FD 2022-08-31
LK http://hdl.handle.net/10668/19971
UL http://hdl.handle.net/10668/19971
LA en
DS RISalud
RD Apr 12, 2025