Publication:
DNA Source Selection for Downstream Applications Based on DNA Quality Indicators Analysis.

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Date

2016-08-01

Authors

Lucena-Aguilar, Gema
Sanchez-Lopez, Ana Maria
Barberan-Aceituno, Cristina
Carrillo-Avila, Jose Antonio
Lopez-Guerrero, Jose Antonio
Aguilar-Quesada, Rocio

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Mary Ann Liebert, Inc. Publishers
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Abstract

High-quality human DNA samples and associated information of individuals are necessary for biomedical research. Biobanks act as a support infrastructure for the scientific community by providing a large number of high-quality biological samples for specific downstream applications. For this purpose, biobank methods for sample preparation must ensure the usefulness and long-term functionality of the products obtained. Quality indicators are the tool to measure these parameters, the purity and integrity determination being those specifically used for DNA. This study analyzes the quality indicators in DNA samples derived from 118 frozen human tissues in optimal cutting temperature (OCT) reactive, 68 formalin-fixed paraffin-embedded (FFPE) tissues, 119 frozen blood samples, and 26 saliva samples. The results obtained for DNA quality are discussed in association with the usefulness for downstream applications and availability of the DNA source in the target study. In brief, if any material is valid, blood is the most approachable option of prospective collection of samples providing high-quality DNA. However, if diseased tissue is a requisite or samples are available, the recommended source of DNA would be frozen tissue. These conclusions will determine the best source of DNA, according to the planned downstream application. Furthermore our results support the conclusion that a complete procedure of DNA quantification and qualification is necessary to guarantee the appropriate management of the samples, avoiding low confidence results, high costs, and a waste of samples.

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MeSH Terms

Biological Specimen Banks
Cryopreservation
DNA
Formaldehyde
Gene Expression Profiling
Humans
Paraffin Embedding
Prospective Studies
Tissue Fixation

DeCS Terms

ADN
Adhesión en parafina
Bancos de muestras biológicas
Criopreservación
Estudios prospectivos
Fijación del tejido
Formaldehído
Humanos
Perfilación de la expresión génica

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Keywords

Biobanking, Biopreservation, Bioprocessing, Cryopreservation, DNA isolation techniques, Forensic genomics, Genetic Engineering, Biotechnology, and Regenerative medicine, Tissue biobanks

Citation

Lucena-Aguilar G, Sánchez-López AM, Barberán-Aceituno C, Carrillo-Ávila JA, López-Guerrero JA, Aguilar-Quesada R. DNA Source Selection for Downstream Applications Based on DNA Quality Indicators Analysis. Biopreserv Biobank. 2016 Aug;14(4):264-70.