RT Journal Article
T1 DNA Source Selection for Downstream Applications Based on DNA Quality Indicators Analysis.
A1 Lucena-Aguilar, Gema
A1 Sanchez-Lopez, Ana Maria
A1 Barberan-Aceituno, Cristina
A1 Carrillo-Avila, Jose Antonio
A1 Lopez-Guerrero, Jose Antonio
A1 Aguilar-Quesada, Rocio
K1 Biobanking
K1 Biopreservation
K1 Bioprocessing
K1 Cryopreservation
K1 DNA isolation techniques
K1 Forensic genomics
K1 Genetic Engineering, Biotechnology, and Regenerative medicine
K1 Tissue biobanks
AB High-quality human DNA samples and associated information of individuals are necessary for biomedical research. Biobanks act as a support infrastructure for the scientific community by providing a large number of high-quality biological samples for specific downstream applications. For this purpose, biobank methods for sample preparation must ensure the usefulness and long-term functionality of the products obtained. Quality indicators are the tool to measure these parameters, the purity and integrity determination being those specifically used for DNA. This study analyzes the quality indicators in DNA samples derived from 118 frozen human tissues in optimal cutting temperature (OCT) reactive, 68 formalin-fixed paraffin-embedded (FFPE) tissues, 119 frozen blood samples, and 26 saliva samples. The results obtained for DNA quality are discussed in association with the usefulness for downstream applications and availability of the DNA source in the target study. In brief, if any material is valid, blood is the most approachable option of prospective collection of samples providing high-quality DNA. However, if diseased tissue is a requisite or samples are available, the recommended source of DNA would be frozen tissue. These conclusions will determine the best source of DNA, according to the planned downstream application. Furthermore our results support the conclusion that a complete procedure of DNA quantification and qualification is necessary to guarantee the appropriate management of the samples, avoiding low confidence results, high costs, and a waste of samples.
PB Mary Ann Liebert, Inc. Publishers
YR 2016
FD 2016-08-01
LK http://hdl.handle.net/10668/10066
UL http://hdl.handle.net/10668/10066
LA en
NO Lucena-Aguilar G, Sánchez-López AM, Barberán-Aceituno C, Carrillo-Ávila JA, López-Guerrero JA, Aguilar-Quesada R. DNA Source Selection for Downstream Applications Based on DNA Quality Indicators Analysis. Biopreserv Biobank. 2016 Aug;14(4):264-70.
DS RISalud
RD Apr 7, 2025