Publication: Metabolic characterization of two different non-alcoholic fatty liver disease pre-clinical mouse models.
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Date
2019
Authors
Gallego-Durán, Rocío
Álvarez-Amor, Leticia
Gil-Gómez, Antonio
Rojas, Ángela
Muñoz-Hernández, Rocío
Cádernas-García, Antonio
Maya-Miles, Douglas
Montero-Vallejo, Rocío
Gato, Sheila
Sánchez Torrijos, Yolanda
Advisors
Journal Title
Journal ISSN
Volume Title
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Abstract
non-alcoholic fatty liver disease is one of the most prevalent liver disorders in the developed world. Currently, there is no approved pharmacological therapy except for lifestyle intervention. Therefore, there is a need to increase the knowledge of preclinical models in order to boost novel discoveries that could lead to a better therapeutic management. this study characterized the effects of two different diets, a long-term high-fat high-fructose diet (HF-HFD) and a choline-deficient, methionine supplemented high-fat diet (CDA-HFD) in C57BL/6J mice for 52 weeks or 16 weeks, respectively. Body weight, lipid and hepatic profile were analyzed and liver histology was subsequently evaluated. HF-HFD animals had an increased body weight and total cholesterol levels, whereas the opposite occurred in CDA-HFD. Both HF-HFD and CDA-HFD animals had higher ALT and AST levels. With regard to histology findings, HF-HFD and CDA-HFD diets induced an increased collagen deposit and intrahepatic steatosis accumulation. in conclusion, the comparison of these models aided in the selection of a long-term, more physiological model for physiopathology studies or a more rapid NASH model for novel molecule testing.
Description
MeSH Terms
Alanine Transaminase
Animals
Aspartate Aminotransferases
Body Weight
Cholesterol
Choline
Diet, High-Fat
Disease Models, Animal
Fructose
Liver
Male
Methionine
Mice
Mice, Inbred C57BL
Non-alcoholic Fatty Liver Disease
Random Allocation
Sweetening Agents
Animals
Aspartate Aminotransferases
Body Weight
Cholesterol
Choline
Diet, High-Fat
Disease Models, Animal
Fructose
Liver
Male
Methionine
Mice
Mice, Inbred C57BL
Non-alcoholic Fatty Liver Disease
Random Allocation
Sweetening Agents