RT Journal Article
T1 Metabolic characterization of two different non-alcoholic fatty liver disease pre-clinical mouse models.
A1 Gallego-Durán, Rocío
A1 Álvarez-Amor, Leticia
A1 Gil-Gómez, Antonio
A1 Rojas, Ángela
A1 Muñoz-Hernández, Rocío
A1 Cádernas-García, Antonio
A1 Maya-Miles, Douglas
A1 Montero-Vallejo, Rocío
A1 Gato, Sheila
A1 Sánchez Torrijos, Yolanda
A1 Ampuero, Javier
A1 Martín, Francisco
A1 Romero-Gómez, Manuel
AB non-alcoholic fatty liver disease is one of the most prevalent liver disorders in the developed world. Currently, there is no approved pharmacological therapy except for lifestyle intervention. Therefore, there is a need to increase the knowledge of preclinical models in order to boost novel discoveries that could lead to a better therapeutic management. this study characterized the effects of two different diets, a long-term high-fat high-fructose diet (HF-HFD) and a choline-deficient, methionine supplemented high-fat diet (CDA-HFD) in C57BL/6J mice for 52 weeks or 16 weeks, respectively. Body weight, lipid and hepatic profile were analyzed and liver histology was subsequently evaluated. HF-HFD animals had an increased body weight and total cholesterol levels, whereas the opposite occurred in CDA-HFD. Both HF-HFD and CDA-HFD animals had higher ALT and AST levels. With regard to histology findings, HF-HFD and CDA-HFD diets induced an increased collagen deposit and intrahepatic steatosis accumulation. in conclusion, the comparison of these models aided in the selection of a long-term, more physiological model for physiopathology studies or a more rapid NASH model for novel molecule testing.
SN 1130-0108
YR 2019
FD 2019
LK http://hdl.handle.net/10668/13743
UL http://hdl.handle.net/10668/13743
LA en
DS RISalud
RD Apr 12, 2025