Publication: Dendrimer-based Uneven Nanopatterns to Locally Control Surface Adhesiveness: A Method to Direct Chondrogenic Differentiation
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Identifiers
Date
2018-01-01
Authors
Casanellas, Ignasi
Lagunas, Anna
Tsintzou, Iro
Vida, Yolanda
Collado, Daniel
Perez-Inestrosa, Ezequiel
Rodriguez-Pereira, Cristina
Magalhaes, Joana
Gorostiza, Pau
Andrades, Jose A.
Advisors
Journal Title
Journal ISSN
Volume Title
Publisher
Journal of visualized experiments
Abstract
Cellular adhesion and differentiation is conditioned by the nanoscale disposition of the extracellular matrix (ECM) components, with local concentrations having a major effect. Here we present a method to obtain large-scale uneven nanopatterns of arginine-glycine-aspartic acid (RGD)-functionalized dendrimers that permit the nanoscale control of local RGD surface density. Nanopatterns are formed by surface adsorption of dendrimers from solutions at different initial concentrations and are characterized by water contact angle (CA), X-ray photoelectron spectroscopy (XPS), and scanning probe microscopy techniques such as scanning tunneling microscopy (STM) and atomic force microscopy (AFM). The local surface density of RGD is measured using AFM images by means of probability contour maps of minimum interparticle distances and then correlated with cell adhesion response and differentiation. The nanopatterning method presented here is a simple procedure that can be scaled up in a straightforward manner to large surface areas. It is thus fully compatible with cell culture protocols and can be applied to other ligands that exert concentration-dependent effects on cells.
Description
MeSH Terms
Adhesiveness
Animals
Cell Adhesion
Chondrogenesis
Dendrimers
Fibroblasts
Mesenchymal Stem Cells
Mice
NIH 3T3 Cells
Nanostructures
Oligopeptides
Surface Properties
Animals
Cell Adhesion
Chondrogenesis
Dendrimers
Fibroblasts
Mesenchymal Stem Cells
Mice
NIH 3T3 Cells
Nanostructures
Oligopeptides
Surface Properties
DeCS Terms
CIE Terms
Keywords
Bioengineering, Issue 131, Dendrimer, Nanopattern, Arginine-Glycine-Aspartic Acid (RGD), Atomic Force Microscopy (AFM), Cell Adhesion, Mesenchymal Stem Cells (Mscs), Chondrogenesis, Cell-adhesion, Stem-cells, D-glucose, Immobilization, Fibronectin, Collagen, Density, Growth