RT Journal Article
T1 Dendrimer-based Uneven Nanopatterns to Locally Control Surface Adhesiveness: A Method to Direct Chondrogenic Differentiation
A1 Casanellas, Ignasi
A1 Lagunas, Anna
A1 Tsintzou, Iro
A1 Vida, Yolanda
A1 Collado, Daniel
A1 Perez-Inestrosa, Ezequiel
A1 Rodriguez-Pereira, Cristina
A1 Magalhaes, Joana
A1 Gorostiza, Pau
A1 Andrades, Jose A.
A1 Becerra, Jose
A1 Samitier, Josep
K1 Bioengineering
K1 Issue 131
K1 Dendrimer
K1 Nanopattern
K1 Arginine-Glycine-Aspartic Acid (RGD)
K1 Atomic Force Microscopy (AFM)
K1 Cell Adhesion
K1 Mesenchymal Stem Cells (Mscs)
K1 Chondrogenesis
K1 Cell-adhesion
K1 Stem-cells
K1 D-glucose
K1 Immobilization
K1 Fibronectin
K1 Collagen
K1 Density
K1 Growth
K1 Centro Andaluz de Nanomedicina y Biotecnología (BIONAND)
AB Cellular adhesion and differentiation is conditioned by the nanoscale disposition of the extracellular matrix (ECM) components, with local concentrations having a major effect. Here we present a method to obtain large-scale uneven nanopatterns of arginine-glycine-aspartic acid (RGD)-functionalized dendrimers that permit the nanoscale control of local RGD surface density. Nanopatterns are formed by surface adsorption of dendrimers from solutions at different initial concentrations and are characterized by water contact angle (CA), X-ray photoelectron spectroscopy (XPS), and scanning probe microscopy techniques such as scanning tunneling microscopy (STM) and atomic force microscopy (AFM). The local surface density of RGD is measured using AFM images by means of probability contour maps of minimum interparticle distances and then correlated with cell adhesion response and differentiation. The nanopatterning method presented here is a simple procedure that can be scaled up in a straightforward manner to large surface areas. It is thus fully compatible with cell culture protocols and can be applied to other ligands that exert concentration-dependent effects on cells.
PB Journal of visualized experiments
SN 1940-087X
YR 2018
FD 2018-01-20
LK http://hdl.handle.net/10668/19339
UL http://hdl.handle.net/10668/19339
LA en
NO Casanellas I, Lagunas A, Tsintzou I, Vida Y, Collado D, Pérez-Inestrosa E, et al. Dendrimer-based Uneven Nanopatterns to Locally Control Surface Adhesiveness: A Method to Direct Chondrogenic Differentiation. J Vis Exp. 2018 Jan 20;(131):56347.
NO The authors acknowledge Oriol Font-Bach and Albert G. Castaño for their help in dmin quantification. They also acknowledge the Advanced Digital Microscopy Unit at the Institute for Research in Biomedicine (IRB Barcelona) to let the authors record the video in their premises. This work was supported by the Networking Biomedical Research Center (CIBER), Spain. CIBER is an initiative funded by the VI National R&D&i Plan 2008-2011, Iniciativa Ingenio 2010, Consolider Program, CIBER Actions, and the Instituto de Salud Carlos III, with the support of the European Regional Development Fund. This work has been supported by the Commission for Universities and Research of the Department of Innovation, Universities, and Enterprise of the Generalitat de Catalunya (2014 SGR 1442). It was also funded by the projects OLIGOCODES (No. MAT2012-38573-C02) and CTQ2013-41339-P, awarded by the Spanish Ministry of Economy and Competitiveness, in addition to INTERREG V-A Spain-Portugal 2014-2020 POCTEP (0245_IBEROS_1_E). C.R.P. acknowledges financial support from the Spanish Ministry of Economy and Competitiveness grant (No. IFI15/00151).
DS RISalud
RD Sep 15, 2025