Publication: Role of c-miR-21, c-miR-126, Redox Status, and Inflammatory Conditions as Potential Predictors of Vascular Damage in T2DM Patients.
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Identifiers
Date
2022-08-19
Authors
Lopez-Armas, Gabriela C
Yessenbekova, Arailym
Gonzalez-Castañeda, Rocio E
Arellano-Arteaga, Kevin J
Guerra-Librero, Ana
Ablaikhanova, Nurzhanyat
Florido, Javier
Escames, Germaine
Acuña-Castroviejo, Darío
Rusanova, Iryna
Advisors
Journal Title
Journal ISSN
Volume Title
Publisher
MDPI AG
Abstract
The development of type 2 diabetes mellitus (T2DM) vascular complications (VCs) is associated with oxidative stress and chronic inflammation and can result in endothelial dysfunctions. Circulating microRNAs play an important role in epigenetic regulation of the etiology of T2DM. We studied 30 healthy volunteers, 26 T2DM patients with no complications, and 26 T2DM patients with VCs, to look for new biomarkers indicating a risk of developing VCs in T2DM patients. Peripheral blood samples were used to determine redox state, by measuring the endogenous antioxidant defense system (superoxide dismutase, SOD; catalase, CAT; glutathione reductase, GRd; glutathione peroxidase, GPx; and glucose-6-phosphate dehydrogenase, G6DP) and markers of oxidative damage (advanced oxidation protein products, AOPP; lipid peroxidation, LPO). Additionally, inflammatory marker levels (IL-1, IL-6, IL-18, and TNF-α), c-miR-21, and c-miR-126 expression were analyzed. T2DM patients showed the highest oxidative damage with increased GSSG/GSH ratios, LPO, and AOPP levels. In both diabetic groups, we found that diminished SOD activity was accompanied by increased CAT and decreased GRd and G6PD activities. Diabetic patients presented with increased relative expression of c-miR-21 and decreased relative expression of c-miR-126. Overall, c-miR-21, SOD, CAT, and IL-6 had high predictive values for diabetes diagnoses. Finally, our data demonstrated that IL-6 exhibited predictive value for VC development in the studied population. Moreover, c-miR-21 and c-miR-126, along with GPx and AOPP levels, should be considered possible markers for VC development in future studies.
Description
MeSH Terms
Antioxidants
MicroRNAs
Advanced Oxidation Protein Products
Diabetes Mellitus, Type 2
Glutathione Reductase
Glutathione Peroxidase
Catalase
Glutathione Disulfide
Interleukin-6
Peroxidase
Epigenesis, Genetic
Interleukin-18
Superoxide Dismutase
Biomarkers
MicroRNAs
Advanced Oxidation Protein Products
Diabetes Mellitus, Type 2
Glutathione Reductase
Glutathione Peroxidase
Catalase
Glutathione Disulfide
Interleukin-6
Peroxidase
Epigenesis, Genetic
Interleukin-18
Superoxide Dismutase
Biomarkers
DeCS Terms
Antioxidantes
Biomarcadores
Catalasa
Diabetes Mellitus tipo 2
Disulfuro de glutatión
Epigénesis genética
Glutatión peroxidasa
Glutatión reductasa
Interleucina-18
Interleucina-6
MicroARNs
Peroxidasa
Productos avanzados de oxidación de proteínas
Superóxido dismutasa
Biomarcadores
Catalasa
Diabetes Mellitus tipo 2
Disulfuro de glutatión
Epigénesis genética
Glutatión peroxidasa
Glutatión reductasa
Interleucina-18
Interleucina-6
MicroARNs
Peroxidasa
Productos avanzados de oxidación de proteínas
Superóxido dismutasa
CIE Terms
Keywords
IL-6, circulating miR-21, miR-126, oxidative stress markers, type 2 diabetes mellitus, vascular complications
Citation
López-Armas GC, Yessenbekova A, González-Castañeda RE, Arellano-Arteaga KJ, Guerra-Librero A, Ablaikhanova N, et al. Role of c-miR-21, c-miR-126, Redox Status, and Inflammatory Conditions as Potential Predictors of Vascular Damage in T2DM Patients. Antioxidants (Basel). 2022 Aug 27;11(9):1675.