RT Journal Article
T1 Role of c-miR-21, c-miR-126, Redox Status, and Inflammatory Conditions as Potential Predictors of Vascular Damage in T2DM Patients.
A1 Lopez-Armas, Gabriela C
A1 Yessenbekova, Arailym
A1 Gonzalez-Castañeda, Rocio E
A1 Arellano-Arteaga, Kevin J
A1 Guerra-Librero, Ana
A1 Ablaikhanova, Nurzhanyat
A1 Florido, Javier
A1 Escames, Germaine
A1 Acuña-Castroviejo, Darío
A1 Rusanova, Iryna
K1 IL-6
K1 circulating miR-21
K1 miR-126
K1 oxidative stress markers
K1 type 2 diabetes mellitus
K1 vascular complications
AB The development of type 2 diabetes mellitus (T2DM) vascular complications (VCs) is associated with oxidative stress and chronic inflammation and can result in endothelial dysfunctions. Circulating microRNAs play an important role in epigenetic regulation of the etiology of T2DM. We studied 30 healthy volunteers, 26 T2DM patients with no complications, and 26 T2DM patients with VCs, to look for new biomarkers indicating a risk of developing VCs in T2DM patients. Peripheral blood samples were used to determine redox state, by measuring the endogenous antioxidant defense system (superoxide dismutase, SOD; catalase, CAT; glutathione reductase, GRd; glutathione peroxidase, GPx; and glucose-6-phosphate dehydrogenase, G6DP) and markers of oxidative damage (advanced oxidation protein products, AOPP; lipid peroxidation, LPO). Additionally, inflammatory marker levels (IL-1, IL-6, IL-18, and TNF-α), c-miR-21, and c-miR-126 expression were analyzed. T2DM patients showed the highest oxidative damage with increased GSSG/GSH ratios, LPO, and AOPP levels. In both diabetic groups, we found that diminished SOD activity was accompanied by increased CAT and decreased GRd and G6PD activities. Diabetic patients presented with increased relative expression of c-miR-21 and decreased relative expression of c-miR-126. Overall, c-miR-21, SOD, CAT, and IL-6 had high predictive values for diabetes diagnoses. Finally, our data demonstrated that IL-6 exhibited predictive value for VC development in the studied population. Moreover, c-miR-21 and c-miR-126, along with GPx and AOPP levels, should be considered possible markers for VC development in future studies.
PB MDPI AG
SN 2076-3921
YR 2022
FD 2022-08-19
LK http://hdl.handle.net/10668/20791
UL http://hdl.handle.net/10668/20791
LA en
NO López-Armas GC, Yessenbekova A, González-Castañeda RE, Arellano-Arteaga KJ, Guerra-Librero A, Ablaikhanova N, et al. Role of c-miR-21, c-miR-126, Redox Status, and Inflammatory Conditions as Potential Predictors of Vascular Damage in T2DM Patients. Antioxidants (Basel). 2022 Aug 27;11(9):1675.
DS RISalud
RD Apr 6, 2025