Publication: Gender-Related Differences on Polyamine Metabolome in Liquid Biopsies by a Simple and Sensitive Two-Step Liquid-Liquid Extraction and LC-MS/MS.
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Identifiers
Date
2019-11-26
Authors
Samarra, Iris
Ramos-Molina, Bruno
Queipo-Ortuño, M Isabel
Tinahones, Francisco J
Arola, Lluís
Delpino-Rius, Antoni
Herrero, Pol
Canela, Núria
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Journal ISSN
Volume Title
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Abstract
Polyamines are involved in the regulation of many cellular functions and are promising biomarkers of numerous physiological conditions. Since the concentrations of these compounds in biological fluids are low, sample extraction is one of the most critical steps of their analysis. Here, we developed a comprehensive, sensitive, robust, and high-throughput LC-MS/MS stable-isotope dilution method for the simultaneous determination of 19 metabolites related to polyamine metabolism, including polyamines, acetylated and diacetylated polyamines, precursors, and catabolites from liquid biopsies. The sample extraction was optimized to remove interfering compounds and to reduce matrix effects, thus being useful for large clinical studies. The method consists of two-step liquid-liquid extraction with a Folch extraction and ethyl acetate partitioning combined with dansyl chloride derivatization. The developed method was applied to a small gender-related trial concerning human serum and urine samples from 40 obese subjects. Sex differences were found for cadaverine, putrescine, 1,3-diaminopropane, γ-aminobutyric acid, N8-acetylspermidine, and N-acetylcadaverine in urine; N1-acetylspermine in serum; and spermine in both serum and urine. The results demonstrate that the developed method can be used to analyze biological samples for the study of polyamine metabolism and its association with human diseases.
Description
MeSH Terms
Acetylation
Cadaverine
Chromatography, Liquid
Dansyl Compounds
Diamines
Female
Humans
Hydrogen-Ion Concentration
Liquid Biopsy
Liquid-Liquid Extraction
Male
Metabolome
Obesity
Polyamines
Putrescine
Sex Characteristics
Spermidine
Spermine
Tandem Mass Spectrometry
gamma-Aminobutyric Acid
Cadaverine
Chromatography, Liquid
Dansyl Compounds
Diamines
Female
Humans
Hydrogen-Ion Concentration
Liquid Biopsy
Liquid-Liquid Extraction
Male
Metabolome
Obesity
Polyamines
Putrescine
Sex Characteristics
Spermidine
Spermine
Tandem Mass Spectrometry
gamma-Aminobutyric Acid
DeCS Terms
CIE Terms
Keywords
LC-MS/MS, acetylpolyamines, liquid biopsies, obesity, polyamine metabolism, polyamines