RT Journal Article
T1 Gender-Related Differences on Polyamine Metabolome in Liquid Biopsies by a Simple and Sensitive Two-Step Liquid-Liquid Extraction and LC-MS/MS.
A1 Samarra, Iris
A1 Ramos-Molina, Bruno
A1 Queipo-Ortuño, M Isabel
A1 Tinahones, Francisco J
A1 Arola, Lluís
A1 Delpino-Rius, Antoni
A1 Herrero, Pol
A1 Canela, Núria
K1 LC-MS/MS
K1 acetylpolyamines
K1 liquid biopsies
K1 obesity
K1 polyamine metabolism
K1 polyamines
AB Polyamines are involved in the regulation of many cellular functions and are promising biomarkers of numerous physiological conditions. Since the concentrations of these compounds in biological fluids are low, sample extraction is one of the most critical steps of their analysis. Here, we developed a comprehensive, sensitive, robust, and high-throughput LC-MS/MS stable-isotope dilution method for the simultaneous determination of 19 metabolites related to polyamine metabolism, including polyamines, acetylated and diacetylated polyamines, precursors, and catabolites from liquid biopsies. The sample extraction was optimized to remove interfering compounds and to reduce matrix effects, thus being useful for large clinical studies. The method consists of two-step liquid-liquid extraction with a Folch extraction and ethyl acetate partitioning combined with dansyl chloride derivatization. The developed method was applied to a small gender-related trial concerning human serum and urine samples from 40 obese subjects. Sex differences were found for cadaverine, putrescine, 1,3-diaminopropane, γ-aminobutyric acid, N8-acetylspermidine, and N-acetylcadaverine in urine; N1-acetylspermine in serum; and spermine in both serum and urine. The results demonstrate that the developed method can be used to analyze biological samples for the study of polyamine metabolism and its association with human diseases.
YR 2019
FD 2019-11-26
LK http://hdl.handle.net/10668/14761
UL http://hdl.handle.net/10668/14761
LA en
DS RISalud
RD Apr 17, 2025