Publication:
Myocardial Ischemic Subject's Thymus Fat: A Novel Source of Multipotent Stromal Cells.

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Date

2015-12-10

Authors

Oliva-Olivera, Wilfredo
Coín-Aragüez, Leticia
Salas, Julián
Lhamyani, Said
Gentile, Adriana-Mariel
Sarria García, Esteban
Hmadcha, Abdelkrim
Zayed, Hatem
Vega-Rioja, Antonio
Tinahones, Francisco J

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Public Libray of Science
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Abstract

OBJECTIVE Adipose Tissue Stromal Cells (ASCs) have important clinical applications in the regenerative medicine, cell replacement and gene therapies. Subcutaneous Adipose Tissue (SAT) is the most common source of these cells. The adult human thymus degenerates into adipose tissue (TAT). However, it has never been studied before as a source of stem cells. MATERIAL AND METHODS We performed a comparative characterization of TAT-ASCs and SAT-ASCs from myocardial ischemic subjects (n = 32) according to the age of the subjects. RESULTS TAT-ASCs and SAT-ASCs showed similar features regarding their adherence, morphology and in their capacity to form CFU-F. Moreover, they have the capacity to differentiate into osteocyte and adipocyte lineages; and they present a surface marker profile corresponding with stem cells derived from AT; CD73+CD90+CD105+CD14-CD19-CD45-HLA-DR. Interestingly, and in opposition to SAT-ASCs, TAT-ASCs have CD14+CD34+CD133+CD45- cells. Moreover, TAT-ASCs from elderly subjects showed higher adipogenic and osteogenic capacities compared to middle aged subjects, indicating that, rather than impairing; aging seems to increase adipogenic and osteogenic capacities of TAT-ASCs. CONCLUSIONS This study describes the human TAT as a source of mesenchymal stem cells, which may have an enormous potential for regenerative medicine.

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Journal Article; Research Support, Non-U.S. Gov't;

MeSH Terms

Medical Subject Headings::Anatomy::Tissues::Connective Tissue::Adipose Tissue
Medical Subject Headings::Named Groups::Persons::Age Groups::Adult::Aged
Medical Subject Headings::Phenomena and Processes::Cell Physiological Phenomena::Cell Physiological Processes::Cell Differentiation
Medical Subject Headings::Phenomena and Processes::Cell Physiological Phenomena::Cell Physiological Processes::Cell Growth Processes::Cell Proliferation
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Clinical Laboratory Techniques::Cytological Techniques::Colony-Forming Units Assay
Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Carrier Proteins::Fatty Acid-Binding Proteins
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Cytological Techniques::Cell Separation::Flow Cytometry
Medical Subject Headings::Phenomena and Processes::Genetic Phenomena::Genetic Processes::Gene Expression
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Clinical Laboratory Techniques::Immunologic Tests::Immunophenotyping
Medical Subject Headings::Anatomy::Cells::Connective Tissue Cells::Stromal Cells::Mesenchymal Stromal Cells
Medical Subject Headings::Named Groups::Persons::Age Groups::Adult::Middle Aged
Medical Subject Headings::Diseases::Cardiovascular Diseases::Heart Diseases::Myocardial Ischemia
Medical Subject Headings::Phenomena and Processes::Musculoskeletal and Neural Physiological Phenomena::Musculoskeletal Physiological Phenomena::Musculoskeletal Physiological Processes::Musculoskeletal Development::Bone Development::Osteogenesis
Medical Subject Headings::Disciplines and Occupations::Health Occupations::Medicine::Regenerative Medicine
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Genetic Techniques::Nucleic Acid Amplification Techniques::Polymerase Chain Reaction::Reverse Transcriptase Polymerase Chain Reaction
Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::DNA-Binding Proteins::Basic-Leucine Zipper Transcription Factors::Basic Helix-Loop-Helix Leucine Zipper Transcription Factors::Sterol Regulatory Element Binding Proteins::Sterol Regulatory Element Binding Protein 1
Medical Subject Headings::Anatomy::Tissues::Connective Tissue::Adipose Tissue::Adipose Tissue, White::Subcutaneous Fat
Medical Subject Headings::Anatomy::Tissues::Lymphoid Tissue::Thymus Gland
Medical Subject Headings::Phenomena and Processes::Cell Physiological Phenomena::Cell Physiological Processes::Cell Differentiation::Adipogenesis

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Keywords

Tejido adiposo, Anciano, Diferenciación celular, Adipogénesis, Proliferación de la célula, Ensayo de unidades formadoras de colonias, Proteínas de unión a ácidos Ggrasos, Citometría de flujo, Expresión génica, Inmunofenotipificación, Células del estroma mesenquimatoso, Mediana edad, Isquemia miocárdica, Osteogénesis, Medicina regenerativa, Reacción en cadena de la polimerasa de transcriptasa inversa, Proteína 1 de unión a los elementos reguladores de esteroles, Grasa subcutánea, Timo

Citation

Oliva-Olivera W, Coín-Aragüez L, Salas J, Lhamyani S, Gentile AM, Sarria García E, et al. Myocardial Ischemic Subject's Thymus Fat: A Novel Source of Multipotent Stromal Cells. PLoS ONE. 2015; 10(12):e0144401