RT Journal Article
T1 Myocardial Ischemic Subject's Thymus Fat: A Novel Source of Multipotent Stromal Cells.
A1 Oliva-Olivera, Wilfredo
A1 Coín-Aragüez, Leticia
A1 Salas, Julián
A1 Lhamyani, Said
A1 Gentile, Adriana-Mariel
A1 Sarria García, Esteban
A1 Hmadcha, Abdelkrim
A1 Zayed, Hatem
A1 Vega-Rioja, Antonio
A1 Tinahones, Francisco J
A1 El Bekay, Rajaa
K1 Tejido adiposo
K1 Anciano
K1 Diferenciación celular
K1 Adipogénesis
K1 Proliferación de la célula
K1 Ensayo de unidades formadoras de colonias
K1 Proteínas de unión a ácidos Ggrasos
K1 Citometría de flujo
K1 Expresión génica
K1 Inmunofenotipificación
K1 Células del estroma mesenquimatoso
K1 Mediana edad
K1 Isquemia miocárdica
K1 Osteogénesis
K1 Medicina regenerativa
K1 Reacción en cadena de la polimerasa de transcriptasa inversa
K1 Proteína 1 de unión a los elementos reguladores de esteroles
K1 Grasa subcutánea
K1 Timo
AB OBJECTIVEAdipose Tissue Stromal Cells (ASCs) have important clinical applications in the regenerative medicine, cell replacement and gene therapies. Subcutaneous Adipose Tissue (SAT) is the most common source of these cells. The adult human thymus degenerates into adipose tissue (TAT). However, it has never been studied before as a source of stem cells.MATERIAL AND METHODSWe performed a comparative characterization of TAT-ASCs and SAT-ASCs from myocardial ischemic subjects (n = 32) according to the age of the subjects.RESULTSTAT-ASCs and SAT-ASCs showed similar features regarding their adherence, morphology and in their capacity to form CFU-F. Moreover, they have the capacity to differentiate into osteocyte and adipocyte lineages; and they present a surface marker profile corresponding with stem cells derived from AT; CD73+CD90+CD105+CD14-CD19-CD45-HLA-DR. Interestingly, and in opposition to SAT-ASCs, TAT-ASCs have CD14+CD34+CD133+CD45- cells. Moreover, TAT-ASCs from elderly subjects showed higher adipogenic and osteogenic capacities compared to middle aged subjects, indicating that, rather than impairing; aging seems to increase adipogenic and osteogenic capacities of TAT-ASCs.CONCLUSIONSThis study describes the human TAT as a source of mesenchymal stem cells, which may have an enormous potential for regenerative medicine.
PB Public Libray of Science
YR 2015
FD 2015-12-10
LK http://hdl.handle.net/10668/2403
UL http://hdl.handle.net/10668/2403
LA en
NO Oliva-Olivera W, Coín-Aragüez L, Salas J, Lhamyani S, Gentile AM, Sarria García E, et al. Myocardial Ischemic Subject's Thymus Fat: A Novel Source of Multipotent Stromal Cells. PLoS ONE. 2015; 10(12):e0144401
NO Journal Article; Research Support, Non-U.S. Gov't;
DS RISalud
RD Apr 11, 2025