Publication: Rapid amplitude-modulation of a diarylethene photoswitch: en route to contrast-enhanced fluorescence imaging
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Date
2021-04-15
Authors
Naren, Gaowa
Larsson, Wera
Benitez-Martin, Carlos
Li, Shiming
Pérez-Inestrosa, Ezequiel
Albinsson, Bo
Andréasson, Joakim
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Publisher
Royal Society of Chemistry
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Abstract
A water soluble diarylethene (DAE) derivative that displays exceptionally intense fluorescence from the colorless open form has been synthesized and characterized using UV/vis spectroscopy and fluorescence microscopy. We show that the bright emission from the open form can be rapidly switched using amplitude modulated red light, that is, by light at wavelengths longer than those absorbed by the fluorescent species. This is highly appealing in any context where undesired background fluorescence disturbs the measurement, e.g., the autofluorescence commonly observed in fluorescence microscopy. We show that this scheme is conveniently applicable using lock-in detection, and that robust amplitude modulation of the probe fluorescence is indeed possible also in cell studies using fluorescence microscopy.
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MeSH Terms
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Microscopy::Microscopy, Fluorescence
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Chemistry Techniques, Analytical::Photometry::Luminescent Measurements::Fluorometry::Spectrometry, Fluorescence
Medical Subject Headings::Chemicals and Drugs::Chemical Actions and Uses::Specialty Uses of Chemicals::Coloring Agents::Fluorescent Dyes
Medical Subject Headings::Phenomena and Processes::Chemical Phenomena::Physicochemical Phenomena::Solubility
Medical Subject Headings::Phenomena and Processes::Physical Phenomena::Optical Phenomena::Light
Medical Subject Headings::Phenomena and Processes::Physical Phenomena::Radiation::Electromagnetic Radiation::Light::Ultraviolet Rays
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Microscopy
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Culture Techniques::Cell Culture Techniques
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Chemistry Techniques, Analytical::Photometry::Luminescent Measurements::Fluorometry::Spectrometry, Fluorescence
Medical Subject Headings::Chemicals and Drugs::Chemical Actions and Uses::Specialty Uses of Chemicals::Coloring Agents::Fluorescent Dyes
Medical Subject Headings::Phenomena and Processes::Chemical Phenomena::Physicochemical Phenomena::Solubility
Medical Subject Headings::Phenomena and Processes::Physical Phenomena::Optical Phenomena::Light
Medical Subject Headings::Phenomena and Processes::Physical Phenomena::Radiation::Electromagnetic Radiation::Light::Ultraviolet Rays
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Microscopy
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Culture Techniques::Cell Culture Techniques
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Keywords
Fluorescence, Microscopy, fluorescence, Fluorescent dyes, UV, Fluorescencia, Microscopía fluorescente, Colorantes fluorescentes, Rayos ultravioleta
Citation
Naren G, Larsson W, Benitez-Martin C, Li S, Pérez-Inestrosa E, Albinsson B, et al. Rapid amplitude-modulation of a diarylethene photoswitch: en route to contrast-enhanced fluorescence imaging. Chem Sci. 2021 Apr 15;12(20):7073-7078