RT Journal Article
T1 Rapid amplitude-modulation of a diarylethene photoswitch: en route to contrast-enhanced fluorescence imaging
A1 Naren, Gaowa
A1 Larsson, Wera
A1 Benitez-Martin, Carlos
A1 Li, Shiming
A1 Pérez-Inestrosa, Ezequiel
A1 Albinsson, Bo
A1 Andréasson, Joakim
K1 Fluorescence
K1 Microscopy, fluorescence
K1 Fluorescent dyes
K1 UV
K1 Fluorescencia
K1 Microscopía fluorescente
K1 Colorantes fluorescentes
K1 Rayos ultravioleta
AB A water soluble diarylethene (DAE) derivative that displays exceptionally intense fluorescence from the colorless open form has been synthesized and characterized using UV/vis spectroscopy and fluorescence microscopy. We show that the bright emission from the open form can be rapidly switched using amplitude modulated red light, that is, by light at wavelengths longer than those absorbed by the fluorescent species. This is highly appealing in any context where undesired background fluorescence disturbs the measurement, e.g., the autofluorescence commonly observed in fluorescence microscopy. We show that this scheme is conveniently applicable using lock-in detection, and that robust amplitude modulation of the probe fluorescence is indeed possible also in cell studies using fluorescence microscopy.
PB Royal Society of Chemistry
YR 2021
FD 2021-04-15
LK http://hdl.handle.net/10668/4165
UL http://hdl.handle.net/10668/4165
LA en
NO Naren G, Larsson W, Benitez-Martin C, Li S, Pérez-Inestrosa E, Albinsson B, et al. Rapid amplitude-modulation of a diarylethene photoswitch: en route to contrast-enhanced fluorescence imaging. Chem Sci. 2021 Apr 15;12(20):7073-7078
DS RISalud
RD Apr 6, 2025