Publication:
New Granada Medium for detection and identification of group B streptococci.

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Date

1992-04

Authors

de la Rosa, Manuel
Perez, Mercedes
Carazo, Concepcion
Pareja, Lucia
Peis, Jose Ignacio
Hernandez, Fernando

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American Society for Microbiology
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Abstract

A methotrexate-containing medium for the detection of beta-hemolytic group B streptococci from clinical specimens on the basis of detection of pigment is described. The medium contained peptone, starch, serum, MgSO4, glucose, pyruvate, methotrexate (as pigment enhancer), phosphate-morpholine-propanesulfonic acid buffer, and selective agents. The recovery of beta-hemolytic group B streptococci was comparable to that obtained with selective broth.

Description

Comparative Study; Journal Article; Research Support, Non-U.S. Gov't;

MeSH Terms

Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Equipment and Supplies::Culture Media
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Evaluation Studies as Topic
Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans
Medical Subject Headings::Chemicals and Drugs::Heterocyclic Compounds::Heterocyclic Compounds, 2-Ring::Pteridines::Pterins::Aminopterin::Methotrexate
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Diagnosis::Diagnostic Techniques and Procedures::Physical Examination::Pigmentation
Medical Subject Headings::Organisms::Bacteria::Gram-Positive Bacteria::Gram-Positive Cocci::Streptococcaceae::Streptococcus::Streptococcus agalactiae
Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Diagnosis::Diagnostic Techniques and Procedures::Clinical Laboratory Techniques::Microbiological Techniques::Bacteriological Techniques

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Keywords

Metotrexato, Medios de cultivo, Pigmentación, Técnicas bacteriológicas, Granada, Andalucía

Citation

de la Rosa M, Perez M, Carazo C, Pareja L, Peis JI, Hernandez F. New Granada Medium for detection and identification of group B streptococci. J Clin Microbiol. 1992; 30(4):1019-21