RT Journal Article T1 New Granada Medium for detection and identification of group B streptococci. A1 de la Rosa, Manuel A1 Perez, Mercedes A1 Carazo, Concepcion A1 Pareja, Lucia A1 Peis, Jose Ignacio A1 Hernandez, Fernando K1 Metotrexato K1 Medios de cultivo K1 Pigmentación K1 Técnicas bacteriológicas K1 Granada K1 Andalucía AB A methotrexate-containing medium for the detection of beta-hemolytic group B streptococci from clinical specimens on the basis of detection of pigment is described. The medium contained peptone, starch, serum, MgSO4, glucose, pyruvate, methotrexate (as pigment enhancer), phosphate-morpholine-propanesulfonic acid buffer, and selective agents. The recovery of beta-hemolytic group B streptococci was comparable to that obtained with selective broth. PB American Society for Microbiology SN 0095-1137 YR 1992 FD 1992-04 LK http://hdl.handle.net/10668/2121 UL http://hdl.handle.net/10668/2121 LA en NO de la Rosa M, Perez M, Carazo C, Pareja L, Peis JI, Hernandez F. New Granada Medium for detection and identification of group B streptococci. J Clin Microbiol. 1992; 30(4):1019-21 NO Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; DS RISalud RD Apr 8, 2025