Publication:
Highly Efficient Neural Conversion of Human Pluripotent Stem Cells in Adherent and Animal-Free Conditions.

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Date

2017-02-18

Authors

Lukovic, Dunja
Diez-Lloret, Andrea
Stojkovic, Petra
Rodriguez-Martinez, Daniel
Perez-Arago, Maria Amparo
Rodriguez-Jimenez, Francisco Javier
Gonzalez-Rodríguez, Patricia
Lopez-Barneo, Jose
Sykova, Eva
Jendelova, Pavla

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Oxford University Press
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Abstract

Neural differentiation of human embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs) can produce a valuable and robust source of human neural cell subtypes, holding great promise for the study of neurogenesis and development, and for treating neurological diseases. However, current hESCs and hiPSCs neural differentiation protocols require either animal factors or embryoid body formation, which decreases efficiency and yield, and strongly limits medical applications. Here we develop a simple, animal-free protocol for neural conversion of both hESCs and hiPSCs in adherent culture conditions. A simple medium formula including insulin induces the direct conversion of >98% of hESCs and hiPSCs into expandable, transplantable, and functional neural progenitors with neural rosette characteristics. Further differentiation of neural progenitors into dopaminergic and spinal motoneurons as well as astrocytes and oligodendrocytes indicates that these neural progenitors retain responsiveness to instructive cues revealing the robust applicability of the protocol in the treatment of different neurodegenerative diseases. The fact that this protocol includes animal-free medium and human extracellular matrix components avoiding embryoid bodies makes this protocol suitable for the use in clinic. Stem Cells Translational Medicine 2017;6:1217-1226.

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Cell Differentiation
Cell- and Tissue-Based Therapy
Cells, Cultured
Embryonic Stem Cells
Humans
Induced Pluripotent Stem Cells
Pluripotent Stem Cells

DeCS Terms

Células madre pluripotentes inducidas
Neurogénesis
Enfermedades neurodegenerativas
Cuerpos embrioides
Insulina
Matriz extracelular
Neuronas motoras

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Keywords

Cellular therapy, Clinical translation, Differentiation, Embryonic stem cells, Induced pluripotent stem cells, Neural differentiation, Pluripotent stem cells

Citation

Lukovic D, Diez Lloret A, Stojkovic P, Rodríguez-Martínez D, Perez Arago MA, Rodriguez-Jimenez FJ, et al. Highly Efficient Neural Conversion of Human Pluripotent Stem Cells in Adherent and Animal-Free Conditions. Stem Cells Transl Med. 2017 Apr;6(4):1217-1226.