RT Journal Article
T1 Highly Efficient Neural Conversion of Human Pluripotent Stem Cells in Adherent and Animal-Free Conditions.
A1 Lukovic, Dunja
A1 Diez Lloret, Andrea
A1 Stojkovic, Petra
A1 Rodríguez-Martínez, Daniel
A1 Perez Arago, Maria Amparo
A1 Rodriguez-Jimenez, Francisco Javier
A1 González-Rodríguez, Patricia
A1 López-Barneo, José
A1 Sykova, Eva
A1 Jendelova, Pavla
A1 Kostic, Jelena
A1 Moreno-Manzano, Victoria
A1 Stojkovic, Miodrag
A1 Bhattacharya, Shomi S
A1 Erceg, Slaven
K1 Cellular therapy
K1 Clinical translation
K1 Differentiation
K1 Embryonic stem cells
K1 Induced pluripotent stem cells
K1 Neural differentiation
K1 Pluripotent stem cells
AB Neural differentiation of human embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs) can produce a valuable and robust source of human neural cell subtypes, holding great promise for the study of neurogenesis and development, and for treating neurological diseases. However, current hESCs and hiPSCs neural differentiation protocols require either animal factors or embryoid body formation, which decreases efficiency and yield, and strongly limits medical applications. Here we develop a simple, animal-free protocol for neural conversion of both hESCs and hiPSCs in adherent culture conditions. A simple medium formula including insulin induces the direct conversion of >98% of hESCs and hiPSCs into expandable, transplantable, and functional neural progenitors with neural rosette characteristics. Further differentiation of neural progenitors into dopaminergic and spinal motoneurons as well as astrocytes and oligodendrocytes indicates that these neural progenitors retain responsiveness to instructive cues revealing the robust applicability of the protocol in the treatment of different neurodegenerative diseases. The fact that this protocol includes animal-free medium and human extracellular matrix components avoiding embryoid bodies makes this protocol suitable for the use in clinic. Stem Cells Translational Medicine 2017;6:1217-1226.
SN 2157-6564
YR 2017
FD 2017-02-18
LK http://hdl.handle.net/10668/10878
UL http://hdl.handle.net/10668/10878
LA en
DS RISalud
RD Apr 11, 2025