Publication:
Quality-controlled ceramide-based GPI-anchored protein sorting into selective ER exit sites.

dc.contributor.authorRodriguez-Gallardo, Sofia
dc.contributor.authorSabido-Bozo, Susana
dc.contributor.authorIkeda, Atsuko
dc.contributor.authorAraki, Misako
dc.contributor.authorOkazaki, Kouta
dc.contributor.authorNakano, Miyako
dc.contributor.authorAguilera-Romero, Auxiliadora
dc.contributor.authorCortes-Gomez, Alejandro
dc.contributor.authorLopez, Sergio
dc.contributor.authorWaga, Miho
dc.contributor.authorNakano, Akihiko
dc.contributor.authorKurokawa, Kazuo
dc.contributor.authorMuñiz, Manuel
dc.contributor.authorFunato, Kouichi
dc.date.accessioned2023-05-03T14:49:43Z
dc.date.available2023-05-03T14:49:43Z
dc.date.issued2022
dc.description.abstractGlycosylphosphatidylinositol-anchored proteins (GPI-APs) exit the endoplasmic reticulum (ER) through a specialized export pathway in the yeast Saccharomyces cerevisiae. We have recently shown that a very-long acyl chain (C26) ceramide present in the ER membrane drives clustering and sorting of GPI-APs into selective ER exit sites (ERES). Now, we show that this lipid-based ER sorting also involves the C26 ceramide as a lipid moiety of GPI-APs, which is incorporated into the GPI anchor through a lipid-remodeling process after protein attachment in the ER. Moreover, we also show that a GPI-AP with a C26 ceramide moiety is monitored by the GPI-glycan remodelase Ted1, which, in turn, is required for receptor-mediated export of GPI-APs. Therefore, our study reveals a quality-control system that ensures lipid-based sorting of GPI-APs into selective ERESs for differential ER export, highlighting the physiological need for this specific export pathway.
dc.description.versionSi
dc.identifier.citationRodriguez-Gallardo S, Sabido-Bozo S, Ikeda A, Araki M, Okazaki K, Nakano M, et al. Quality-controlled ceramide-based GPI-anchored protein sorting into selective ER exit sites. Cell Rep. 2022 May 3;39(5):110768.
dc.identifier.doi10.1016/j.celrep.2022.110768
dc.identifier.essn2211-1247
dc.identifier.pmid35508142
dc.identifier.unpaywallURLhttp://www.cell.com/article/S2211124722005320/pdf
dc.identifier.urihttp://hdl.handle.net/10668/22076
dc.issue.number5
dc.journal.titleCell reports
dc.journal.titleabbreviationCell Rep
dc.language.isoen
dc.organizationHospital Universitario Virgen del Rocío
dc.organizationInstituto de Biomedicina de Sevilla-IBIS
dc.page.number16
dc.publisherCell Press
dc.pubmedtypeJournal Article
dc.relation.publisherversionhttps://linkinghub.elsevier.com/retrieve/pii/S2211-1247(22)00532-0
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International
dc.rights.accessRightsopen access
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectCP: Cell biology
dc.subjectCP: Molecular biology
dc.subjectGPI-anchored protein
dc.subjectceramide remodeling
dc.subjectendoplasmic reticulum
dc.subjectglycan remodeling
dc.subjectprotein sorting
dc.subjectquality control
dc.subjectyeast Saccharomyces cerevisiae
dc.subject.decsLípidos
dc.subject.decsCeramidas
dc.subject.decsProteínas
dc.subject.decsGlicosilfosfatidilinositoles
dc.subject.decsSaccharomyces cerevisiae
dc.subject.decsAnálisis por conglomerados
dc.subject.meshCeramides
dc.subject.meshEndoplasmic Reticulum
dc.subject.meshGPI-Linked Proteins
dc.subject.meshGlycosylphosphatidylinositols
dc.subject.meshProtein Transport
dc.subject.meshSaccharomyces cerevisiae
dc.titleQuality-controlled ceramide-based GPI-anchored protein sorting into selective ER exit sites.
dc.typeresearch article
dc.type.hasVersionVoR
dc.volume.number39
dspace.entity.typePublication

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