Publication:
Quality-controlled ceramide-based GPI-anchored protein sorting into selective ER exit sites.

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2022

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Rodriguez-Gallardo, Sofia
Sabido-Bozo, Susana
Ikeda, Atsuko
Araki, Misako
Okazaki, Kouta
Nakano, Miyako
Aguilera-Romero, Auxiliadora
Cortes-Gomez, Alejandro
Lopez, Sergio
Waga, Miho

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Abstract

Glycosylphosphatidylinositol-anchored proteins (GPI-APs) exit the endoplasmic reticulum (ER) through a specialized export pathway in the yeast Saccharomyces cerevisiae. We have recently shown that a very-long acyl chain (C26) ceramide present in the ER membrane drives clustering and sorting of GPI-APs into selective ER exit sites (ERES). Now, we show that this lipid-based ER sorting also involves the C26 ceramide as a lipid moiety of GPI-APs, which is incorporated into the GPI anchor through a lipid-remodeling process after protein attachment in the ER. Moreover, we also show that a GPI-AP with a C26 ceramide moiety is monitored by the GPI-glycan remodelase Ted1, which, in turn, is required for receptor-mediated export of GPI-APs. Therefore, our study reveals a quality-control system that ensures lipid-based sorting of GPI-APs into selective ERESs for differential ER export, highlighting the physiological need for this specific export pathway.

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Ceramides
Endoplasmic Reticulum
GPI-Linked Proteins
Glycosylphosphatidylinositols
Protein Transport
Saccharomyces cerevisiae

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Keywords

CP: Cell biology, CP: Molecular biology, GPI-anchored protein, ceramide remodeling, endoplasmic reticulum, glycan remodeling, protein sorting, quality control, yeast Saccharomyces cerevisiae

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