Amigo-Benavent, MiryamSinausia, LauraMontero, EmilioPerona, Javier S2023-01-252023-01-252016-03-17http://hdl.handle.net/10668/9930The atherogenicity of triglyceride-rich lipoproteins (TRLs) is dependent of their particle size as it determines their metabolic fate. Since TRL possess a single apolipoprotein B (Apo B) molecule per particle, the triglyceride (TG)/Apo B ratio has been used as a convenient method to estimate TRL size. The aim of this study was to validate this approach by correlating the serum TG/Apo B ratio, and the TRL particle size measured by dynamic light scattering (DLS). Twenty-four male volunteers (12 normal-weight and 12 obese individuals) received a high-fat meal. Preprandial (0 h) and postprandial (2 and 4 h) serum samples were collected after meal ingestion, and TRLs were isolated. Serum TG and Apo B levels were quantified, and the TG/Apo B ratio was plotted against TRL particle size measured by DLS for correlation. A strong association between TRL particle size and serum TG/Apo B ratio for normal-weight subjects (P ≤ 0.001) was observed but not for obese subjects (P = 0.6116). TG/Apo B ratio correlates with particle size in healthy normal-weight males but not in obese individuals. Whether this ratio is useful to estimate TRL size in females and in other dyslipidemic patients should be subject of future investigations.enApolipoprotein Bparticle sizeratiotriglyceridetriglyceride-rich lipoproteinAdultApolipoproteins BBody Mass IndexCase-Control StudiesDietary FatsHumansLipoproteinsMaleObesityParticle SizeTriglyceridesresearch article26993672open access10.1177/15353702166393941535-3699PMC5027931https://europepmc.org/articles/pmc5027931?pdf=renderhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5027931/pdf