Salguero-Aranda, CarmenAmaral, Ana TeresaOlmedo-Pelayo, JoaquínDiaz-Martin, Juande Álava, Enrique2022-07-222022-07-222020-03-26Salguero-Aranda C, Amaral AT, Olmedo-Pelayo J, Diaz-Martin J, de Álava E. Breakthrough Technologies Reshape the Ewing Sarcoma Molecular Landscape. Cells. 2020 Mar 26;9(4):804http://hdl.handle.net/10668/3811Ewing sarcoma is a highly aggressive round cell mesenchymal neoplasm, most often occurring in children and young adults. At the molecular level, it is characterized by the presence of recurrent chromosomal translocations. In the last years, next-generation technologies have contributed to a more accurate diagnosis and a refined classification. Moreover, the application of these novel technologies has highlighted the relevance of intertumoral and intratumoral molecular heterogeneity and secondary genetic alterations. Furthermore, they have shown evidence that genomic features can change as the tumor disseminates and are influenced by treatment as well. Similarly, next-generation technologies applied to liquid biopsies will significantly impact patient management by allowing the early detection of relapse and monitoring response to treatment. Finally, the use of these novel technologies has provided data of great value in order to discover new druggable pathways. Thus, this review provides concise updates on the latest progress of these breakthrough technologies, underscoring their importance in the generation of key knowledge, prognosis, and potential treatment of Ewing Sarcoma.enAtribución 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/SarcomaEwing sarcomaGFExtracellular vesiclesCirculating tumor DNA/RNADNA repairBreakthrough technologiesMutationsSarcoma de EwingFusión génicaVesículas extracelularesADN tumoral circulanteReparación del ADNMutaciónSecuenciación de nucleótidos de alto rendimientoMedical Subject Headings::Organisms::Eukaryota::AnimalsMedical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::HumansMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Therapeutics::Drug Therapy::Molecular Targeted TherapyMedical Subject Headings::Phenomena and Processes::Genetic Phenomena::PhenotypeMedical Subject Headings::Diseases::Neoplasms::Neoplasms by Histologic Type::Neoplasms, Connective and Soft Tissue::Neoplasms, Connective Tissue::Neoplasms, Bone Tissue::Osteosarcoma::Sarcoma, EwingMedical Subject Headings::Diseases::Neoplasms::Neoplasms by Histologic Type::Neoplasms, Connective and Soft Tissue::SarcomaMedical Subject Headings::Phenomena and Processes::Genetic Phenomena::Genetic Processes::DNA RepairMedical Subject Headings::Phenomena and Processes::Genetic Phenomena::Genetic Variation::MutationMedical Subject Headings::Persons::Persons::Age Groups::Adult::Young AdultMedical Subject Headings::Persons::Persons::Age Groups::ChildMedical Subject Headings::Phenomena and Processes::Genetic Phenomena::Genetic Processes::Mutagenesis::Translocation, GeneticMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Genetic Techniques::Sequence Analysis::High-Throughput Nucleotide SequencingMedical Subject Headings::Diseases::Pathological Conditions, Signs and Symptoms::Pathologic Processes::Disease Attributes::Recurrenceresearch article32225029open access10.3390/cells90408042073-4409PMC7226764