Gonzalo-Gobernado, RafaelAyuso, María IreneSansone, LoredanaBernal-Jiménez, Juan JoséRamos-Herrero, Víctor DaríoSánchez-García, EnriqueRamos, Teresa LAbia, RocíoMuriana, Francisco J GBermúdez, BeatrizMontaner, Joan2023-01-252023-01-252019-05-18http://hdl.handle.net/10668/13991Stroke is one of the leading causes of death worldwide and while there is increasing evidence that a Mediterranean diet might decrease the risk of a stroke, the effects of dietary fat composition on stroke outcomes have not been fully explored. We hypothesize that the brain damage provoked by a stroke would be different depending on the source of dietary fat. To test this, male C57BL/6J mice were fed for 4 weeks with a standard low-fat diet (LFD), a high-fat diet (HFD) rich in saturated fatty acids (HFD-SFA), an HFD containing monounsaturated fatty acids (MUFAs) from olive oil (HFD-OO), or an HFD containing MUFAs from olive oil plus polyunsaturated fatty acids (PUFAs) docosahexaenoic acid/eicosapentaenoic acid (DHA/EPA) (HFD-OO-ω3). These mice were then subjected to transient middle cerebral artery occlusion (tMCAo). Behavioural tests and histological analyses were performed 24 and/or 48 h after tMCAo in order to elucidate the impact of these diets with different fatty acid profiles on the ischemic lesion and on neurological functions. Mice fed with HFD-OO-ω3 displayed better histological outcomes after cerebral ischemia than mice that received an HFD-SFA or LFD. Furthermore, PUFA- and MUFA-enriched diets improved the motor function and neurological performance of ischemic mice relative to those fed with an LFD or HFD-SFA. These findings support the use of DHA/EPA-omega-3-fatty acid supplementation and olive oil as dietary source of MUFAs in order to reduce the damage and protect the brain when a stroke occurs.enAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/DHAEPAMUFAPUFASFAbehaviourcerebral ischemiamiddle cerebral artery occlusion (MCAo)neurological functionneuroprotectionolive oilomega-3 fatty acidsstrokeAnimalsAntioxidantsBehavior, AnimalBrain IschemiaDiet, High-FatDocosahexaenoic AcidsEatingEicosapentaenoic AcidGaitGene Expression Regulation, EnzymologicMaleMiceMice, Inbred C57BLMiddle Cerebral ArteryOlive OilWeight Lossresearch article31109078open access10.3390/nu110511092072-6643PMC6566717https://www.mdpi.com/2072-6643/11/5/1109/pdf?version=1558173255https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6566717/pdf