Pérez-Ruiz, MercedesPedrosa-Corral, IreneSanbonmatsu-Gámez, SaraNavarro-Marí, María2013-04-182013-04-182012-11-30Pérez-Ruiz M, Pedrosa-Corral I, Sanbonmatsu-Gámez S, Navarro-Marí M. Laboratory detection of respiratory viruses by automated techniques. Open Virol J 2009; 6:151-9http://hdl.handle.net/10668/907Journal Article; Se utiliza la url que lleva al articulo en pdf porque la url del abstract es incorrecta.Advances in clinical virology for detecting respiratory viruses have been focused on nucleic acids amplification techniques, which have converted in the reference method for the diagnosis of acute respiratory infections of viral aetiology. Improvements of current commercial molecular assays to reduce hands-on-time rely on two strategies, a stepwise automation (semi-automation) and the complete automation of the whole procedure. Contributions to the former strategy have been the use of automated nucleic acids extractors, multiplex PCR, real-time PCR and/or DNA arrays for detection of amplicons. Commercial fully-automated molecular systems are now available for the detection of respiratory viruses. Some of them could convert in point-of-care methods substituting antigen tests for detection of respiratory syncytial virus and influenza A and B viruses. This article describes laboratory methods for detection of respiratory viruses. A cost-effective and rational diagnostic algorithm is proposed, considering technical aspects of the available assays, infrastructure possibilities of each laboratory and clinic-epidemiologic factors of the infection.enAcute respiratory infectionAutomationInfluenza-like illnessmicroarrayReal-time PCRRespiratory virusesInfecciones del Sistema RespiratorioAutomatizaciónAnálisis por MicromatricesReacción en Cadena en Tiempo Real de la PolimerasaMedical Subject Headings::Diseases::Respiratory Tract Diseases::Respiratory Tract InfectionsMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Automation, LaboratoryMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Microchip Analytical Procedures::Microarray AnalysisMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Genetic Techniques::Nucleic Acid Amplification Techniques::Polymerase Chain Reaction::Real-Time Polymerase Chain Reactionresearch article23248735open access10.2174/18743579012060101511874-3579PMC3522051