Moreno-Morales, JavierVergara, AndreaKostyanev, TomislavRodriguez-Baño, JesúsGoossens, HermanVila, Jordi2022-09-082022-09-082021-01-13Moreno-Morales J, Vergara A, Kostyanev T, Rodriguez-Baño J, Goossens H, Vila J. Evaluation of a Loop-Mediated Isothermal Amplification Assay to Detect Carbapenemases Directly From Bronchoalveolar Lavage Fluid Spiked With Acinetobacter spp. Front Microbiol. 2021 Jan 13;11:597684http://hdl.handle.net/10668/4020Carbapenem-resistant Acinetobacter spp. mainly Acinetobacter baumannii are frequently causing nosocomial infections with high mortality. In this study, the efficacy of the Eazyplex® SuperBug Complete A system, based on loop-mediated isothermal amplification (LAMP), to detect the presence of carbapenemases in Acinetobacter spp. directly from bronchoalveolar lavage (BAL) samples was assessed. A total of 22 Acinetobacter spp. strains producing OXA-23, OXA-40, OXA-58, NDM, and IMP were selected. Eazyplex SuperBug Complete A kit, used with the Genie II device, is a molecular diagnostics kit that detects a selection of genes that express carbapenemases (bla KPC , bla NDM , bla VIM , bla OXA-48 , bla OXA-23 , bla OXA-40 , and bla OXA-58 ). Negative BAL samples were identified, McFarland solutions were prepared from each of the 22 Acinetobacter strains and serial dilutions in saline solution were made to finally spike BAL samples to a concentration of 102 and 103 CFU/ml. Fifteen concentrations out of the 44 tested out did not provide detection of the carbapenemase-producing gene, all but one being at the lowest concentration tested at 102 CFU/ml; therefore, the limit of sensitivity is 103 CFU/ml. This assay represents the kind of advantages that investing in molecular diagnostics brings to the clinical practice, allowing the identification of carbapenemases in less than 30 min with a sensitivity of 103 CFU/ml.enAtribución 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/CarbapenemasesAcinetobacter sppBronchoalveolar lavageDetectionOxacillinasesMedical Subject Headings::Organisms::Bacteria::Gram-Negative Bacteria::Gram-Negative Aerobic Bacteria::Gram-Negative Aerobic Rods and Cocci::Moraxellaceae::Acinetobacter::Acinetobacter baumanniiMedical Subject Headings::Chemicals and Drugs::Heterocyclic Compounds::Heterocyclic Compounds, 2-Ring::Purines::Purine Nucleotides::Inosine Nucleotides::Inosine MonophosphateMedical Subject Headings::Chemicals and Drugs::Pharmaceutical Preparations::Solutions::Hypertonic Solutions::Saline Solution, HypertonicMedical Subject Headings::Diseases::Bacterial Infections and Mycoses::Infection::Cross InfectionMedical Subject Headings::Disciplines and Occupations::Natural Science Disciplines::Biological Science Disciplines::Biochemistry::Molecular Biology::Molecular Medicine::Pathology, MolecularMedical Subject Headings::Chemicals and Drugs::Organic Chemicals::Amides::Lactams::beta-Lactams::CarbapenemsMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Therapeutic Irrigation::Bronchoalveolar Lavageresearch article33519735open accessEnterobacterias resistentes a carbapenémicosLavado broncoalveolarDiagnósticoOxacilina10.3389/fmicb.2020.5976841664-3224PMC7838357