Lorente-Macías, ÁlvaroIañez, InmaculadaJiménez-López, M CarmenBenítez-Quesada, ManuelTorres-Rusillo, SaraDíaz-Mochón, Juan JMolina, Ignacio JPineda de Las Infantas, María J2025-01-072025-01-072021-06-15https://hdl.handle.net/10668/27669Purines are ubiquitous structures in cell biology involved in a multitude of cellular processes, because of which substituted purines and analogs are considered excellent scaffolds in drug design. In this study, we explored the key structural features of a purine-based proapoptotic hit, 8-tert-butyl-9-phenyl-6-benzyloxy-9H-purine (1), by setting up a library of 6-alkoxy purines with the aim of elucidating the structural requirements that govern its biological activity and to study the cell selectivity of this chemotype. This was done by a phenotypic screening approach based on cell cycle analysis of a panel of six human cancer cell lines, including T cell leukemia Jurkat cells. From this study, two derivatives (12 and 13) were identified as Jurkat-selective proapoptotic compounds, displaying superior potency and cell selectivity than hit 1.enAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/anticancer drugsapoptosisleukemiaphenotypic screeningpurine analogsAntineoplastic AgentsApoptosisCell Line, TumorHumansJurkat CellsLeukemia, T-CellPurinesStructure-Activity Relationshipresearch article34128249open access10.1002/ardp.2021000951521-4184https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/ardp.202100095