Ravoitytė, BazilėLukša, JulianaWellinger, Ralf ErikServa, SauliusServienė, Elena2023-05-032023-05-032022-04-09http://hdl.handle.net/10668/21368Totiviridae L-A virus is a widespread yeast dsRNA virus. The persistence of the L-A virus alone appears to be symptomless, but the concomitant presence of a satellite M virus provides a killer trait for the host cell. The presence of L-A dsRNA is common in laboratory, industrial, and wild yeasts, but little is known about the impact of the L-A virus on the host's gene expression. In this work, based on high-throughput RNA sequencing data analysis, the impact of the L-A virus on whole-genome expression in three different Saccharomyces paradoxus and S. cerevisiae host strains was analyzed. In the presence of the L-A virus, moderate alterations in gene expression were detected, with the least impact on respiration-deficient cells. Remarkably, the transcriptional adaptation of essential genes was limited to genes involved in ribosome biogenesis. Transcriptional responses to L-A maintenance were, nevertheless, similar to those induced upon stress or nutrient availability. Based on these data, we further dissected yeast transcriptional regulators that, in turn, modulate the cellular L-A dsRNA levels. Our findings point to totivirus-driven fine-tuning of the transcriptional landscape in yeasts and uncover signaling pathways employed by dsRNA viruses to establish the stable, yet allegedly profitless, viral infection of fungi.enAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/Saccharomyces cerevisiaeSaccharomyces paradoxusTotiviridaedsRNAtranscriptionyeastresearch article35448612open access10.3390/jof80403812309-608XPMC9028071https://www.mdpi.com/2309-608X/8/4/381/pdf?version=1649653247https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9028071/pdf