Schröder, HelmutSubirana, IsaacWärnberg, JuliaMedrano, MaríaGonzález-Gross, MarcelaGusi, NarcisAznar, SusanaAlcaraz, Pedro EGonzález-Valeiro, Miguel ASerra-Majem, LluisTerrados, NicolásTur, Josep ASegú, MartaHoms, ClaraGarcia-Álvarez, AliciaBenavente-Marín, Juan CBarón-López, F JavierLabayen, IdoiaZapico, Augusto GSánchez-Gómez, JesúsJiménez-Zazo, FabioMarín-Cascales, ElenaSevilla-Sanchez, MartaHerrera-Ramos, EstefaníaPulgar, SusanaDel Mar Bibiloni, MaríaSistac-Sorigué, ClaraGómez, Santiago F2023-02-092023-02-092021-07-17http://hdl.handle.net/10668/18211Validation of self-reported tools, such as physical activity (PA) questionnaires, is crucial. The aim of this study was to determine test-retest reliability, internal consistency, and the concurrent, construct, and predictive validity of the short semi-quantitative Physical Activity Unit 7 item Screener (PAU-7S), using accelerometry as the reference measurement. The effect of linear calibration on PAU-7S validity was tested. A randomized sample of 321 healthy children aged 8-16 years (149 boys, 172 girls) from the nationwide representative PASOS study completed the PAU-7S before and after wearing an accelerometer for at least 7 consecutive days. Weight, height, and waist circumference were measured. Cronbach alpha was calculated for internal consistency. Test-retest reliability was determined by intra-class correlation (ICC). Concurrent validity was assessed by ICC and Spearman correlation coefficient between moderate to vigorous PA (MVPA) derived by the PAU-7S and by accelerometer. Concordance between both methods was analyzed by absolute agreement, weighted kappa, and Bland-Altman statistics. Multiple linear regression models were fitted for construct validity and predictive validity was determined by leave-one-out cross-validation. The PAU-7S overestimated MVPA by 18%, compared to accelerometers (106.5 ± 77.0 vs 95.2 ± 33.2 min/day, respectively). A Cronbach alpha of 0.76 showed an acceptable internal consistency of the PAU-7S. Test-retest reliability was good (ICC 0.71 p  The PAU-7S shows a good test-retest reliability and acceptable internal consistency. All dimensions of validity increased from poor/fair to moderate/good after calibration. The PAU-7S is a valid instrument for measuring MVPA in children and adolescents. Trial registration number ISRCTN34251612 .enAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/AccelerometryChildren and adolescentsSelf-reported physical activityShort PAQValidationAccelerometryAdolescentCalibrationChildExerciseFemaleHumansMaleReproducibility of ResultsSedentary BehaviorSurveys and Questionnairesresearch article34274002open access10.1186/s12966-021-01169-w1479-5868PMC8285783https://ijbnpa.biomedcentral.com/counter/pdf/10.1186/s12966-021-01169-whttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8285783/pdf