Valhondo, IsabelHassouneh, FakhriLopez-Sejas, NelsonPera, AlejandraSanchez-Correa, BeatrizGuerrero, BeatrizBergua, Juan MArcos, Maria JoseBañas, HelenaCasas-Avilés, IgnacioSanchez-Garcia, JoaquinSerrano, JosefinaMartin, CarmenDuran, EstherAlonso, CoronaSolana, RafaelTarazona, Raquel2025-01-072025-01-072020-08-052072-6694https://hdl.handle.net/10668/25804Background: Acute myeloid leukemia (AML) remains a major clinical challenge due to poor overall survival, which is even more dramatic in elderly patients. TIGIT, an inhibitory receptor that interacts with CD155 and CD112 molecules, is considered as a checkpoint in T and NK cell activation. This receptor shares ligands with the co-stimulatory receptor DNAM-1 and with TACTILE. The aim of this work was to analyze the expression of DNAM-1, TIGIT and TACTILE in NK cells and T cell subsets in AML patients. Methods: We have studied 36 patients at the time of diagnosis of AML and 20 healthy volunteers. The expression of DNAM-1, TIGIT and TACTILE in NK cells and T cells, according to the expression of CD3 and CD56, was performed by flow cytometry. Results: NK cells, CD56- T cells and CD56+ T (NKT-like) cells from AML patients presented a reduced expression of DNAM-1 compared with healthy volunteers. An increased expression of TIGIT was observed in mainstream CD56- T cells. No differences were observed in the expression of TACTILE. Simplified presentation of incredibly complex evaluations (SPICE) analysis of the co-expression of DNAM-1, TIGIT and TACTILE showed an increase in NK and T cells lacking DNAM-1 and co-expressing TIGIT and TACTILE. Low percentages of DNAM-1-TIGIT+TACTILE+ NK cells and DNAM-1- TIGIT+TACTILE+ CD56- T cells were associated with a better survival of AML patients. Conclusions: The expression of DNAM-1 is reduced in NK cells and in CD4+ and CD8+ T cells from AML patients compared with those from healthy volunteers. An increased percentage of NK and T cells lacking DNAM-1 and co-expressing TIGIT and TACTILE is associated with patient survival, supporting the role of TIGIT as a novel candidate for checkpoint blockade.enAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/DNAM-1NK cellsNKT-like cellsT cellsTACTILETIGITacute myeloid leukemiaresearch article32764229open access10.3390/cancers12082171PMC7464787https://www.mdpi.com/2072-6694/12/8/2171/pdf?version=1597039439https://pmc.ncbi.nlm.nih.gov/articles/PMC7464787/pdf