Santos, CristinaGarcia-Fuentes, EduardoCallejón-Leblic, BelenGarcia-Barrera, TamaraGomez-Ariza, Jose LuisRayman, Margaret PVelasco, Ines2023-01-252023-01-252017-05-23Santos C, García-Fuentes E, Callejón-Leblic B, García-Barrera T, Gómez-Ariza JL, Rayman MP, et al. Selenium, selenoproteins and selenometabolites in mothers and babies at the time of birth. Br J Nutr. 2017 May;117(9):1304-1311http://hdl.handle.net/10668/11221The deficiency of Se, an essential micronutrient, has been implicated in adverse pregnancy outcomes. Our study was designed to determine total serum Se, selenoproteins (extracellular glutathione peroxidase (GPx-3), selenoprotein P (SeP)), selenoalbumin (SeAlb) and selenometabolites in healthy women and their newborns at delivery. This cross-sectional study included eighty-three healthy mother-baby couples. Total Se and Se species concentrations were measured in maternal and umbilical cord sera by an in-series coupling of two-dimensional size-exclusion and affinity HPLC. Additional measurements of serum SeP concentration and of serum GPx-3 enzyme activity were carried out using ELISA. Total Se concentration was significantly higher in maternal serum than in cord serum (68·9 (sd 15·2) and 56·1 (sd 14·6) µg/l, respectively; P<0·01). There were significant correlations between selenoprotein and SeAlb concentrations in mothers and newborns, although they also showed significant differences in GPx-3 (11·2(SD 3·7) v.10·5(SD 3·5)µg/l; P<0·01), SeP (42·5(SD 9·5) v.28·1 (SD 7·7)µg/l; P<0·01) and SeAlb (11·6(SD 3·6) v.14·1(SD 4·3)µg/l; P<0·01) concentrations in maternal and cord sera, respectively. Serum GPx-3 activity and concentration were positively correlated in mothers (r 0·33; P=0·038) but not in newborns. GPx-3 activity in cord serum was significantly correlated with gestational age (r 0·44; P=0·009). SeAlb concentration was significantly higher in babies, whereas SeP and GPx-3 concentrations were significantly higher in mothers. The differences cannot be explained by simple diffusion; specific transfer mechanisms are probably involved. GPx-3 concentrations in mothers, at delivery, are related to maternal Se status, whereas the GPx-3 activity in cord serum depends on gestational age.en2D/SE-AF-HPLC two-dimensional size-exclusion and affinity HPLCGPx-3 extracellular glutathione peroxidaseSeAlb selenoalbuminSeMetab selenometabolitesSeP selenoprotein PGestationSeleniumSelenoprotein PSelenoproteinsÁrea de Gestión Sanitaria Norte de HuelvaAdultCross-Sectional StudiesEnzyme-Linked Immunosorbent AssayFemaleFetal BloodGlutathione PeroxidaseHumansInfant, NewbornPostpartum PeriodPregnancySelenoproteinsYoung Adultresearch article28534447Restricted AccessEdad gestacionalSelenoproteínasComposición familiarMicronutrientesCromatografía líquida de alta presiónEnsayo de inmunoadsorción enzimática10.1017/S00071145170011551475-2662https://www.cambridge.org/core/services/aop-cambridge-core/content/view/CB9FFB9C9E27E06234202A572492D4A7/S0007114517001155a.pdf/div-class-title-selenium-selenoproteins-and-selenometabolites-in-mothers-and-babies-at-the-time-of-birth-div.pdf