Vergara, AMoreno-Morales, JRoca, IPitart, CKostyanev, TRodriguez-Baño, JGoossens, HMarco, FVila, J2023-02-082023-02-082020-02-19Vergara A, Moreno-Morales J, Roca I, Pitart C, Kostyanev T, Rodriguez-Baño J, et al. A comparative study between real-time PCR and loop-mediated isothermal amplification to detect carbapenemase and/or ESBL genes in Enterobacteriaceae directly from bronchoalveolar lavage fluid samples. J Antimicrob Chemother. 2020 Jun 1;75(6):1453-1457http://hdl.handle.net/10668/15128To evaluate and compare the efficacy of real-time PCR (Xpert Carba-R) and loop-mediated isothermal amplification (Eazyplex® SuperBug CRE) for detecting carbapenemase carriage in Enterobacteriaceae directly from bronchoalveolar lavage (BAL). Negative BAL samples were spiked with 21 well-characterized carbapenemase-producing Enterobacteriaceae strains to a final concentration of 102-104 cfu/mL. Xpert Carba-R (Cepheid, Sunnyvale, CA, USA), which detects five targets (blaKPC, blaNDM, blaVIM, blaOXA-48 and blaIMP-1), and the Eazyplex® SuperBug CRE system (Amplex-Diagnostics GmbH, Germany), which detects seven genes (blaKPC, blaNDM, blaVIM, blaOXA-48, blaOXA-181, blaCTXM-1 and blaCTXM-9), were evaluated for the detection of these genes directly from BAL samples. Xpert Carba-R showed 100% agreement with carbapenemase characterization by PCR and sequencing for all final bacteria concentrations. Eazyplex® SuperBug CRE showed 100%, 80% and 27% agreement with PCR and sequencing when testing 104, 103 and 102 cfu/mL, respectively. False negative results for Eazyplex® SuperBug CRE matched the highest cycle threshold values for Xpert Carba-R. Hands-on time for both assays was about 15 min, but Eazyplex® SuperBug CRE results were available within 30 min, whereas Xpert Carba-R took around 50 min. We here describe the successful use of two commercial diagnostic tests, Xpert Carba-R and Eazyplex® SuperBug CRE, to detect bacterial carbapenem resistance genes directly in lower respiratory tract samples. Our results could be used as proof-of-concept data for validation of these tests for this indication.enBronchoalveolar Lavage FluidGermanyNucleic Acid Amplification TechniquesSensitivity and SpecificityBacterial ProteinsEnterobacteriaceaeMolecular Diagnostic TechniquesReal-Time Polymerase Chain Reactionbeta-Lactamasesresearch article32073602Restricted AccessGenesEnterobacteriaceaeReacción en cadena en tiempo real de la polimerasaResultados negativosSistema respiratorioEsguinces y distensionesLavado broncoalveolar10.1093/jac/dkaa0311460-2091http://diposit.ub.edu/dspace/bitstream/2445/176218/1/707110.pdf