Cirit, Osman SezerFernandez-Martinez, MartaYayla, BuketMartinez-Martinez, Luis2023-01-252023-01-252019-02-26Cirit OS, Fernández-Martínez M, Yayla B, Martínez-Martínez L. Aminoglycoside resistance determinants in multiresistant Escherichia coli and Klebsiella pneumoniae clinical isolates from Turkish and Syrian patients. Acta Microbiol Immunol Hung. 2019 Sep 1;66(3):327-335http://hdl.handle.net/10668/13625Escherichia coli and Klebsiella pneumoniae are frequently found resistance to aminoglycosides in Turkey. The aim of this study was to investigate aminoglycoside resistance in clinical isolates of E. coli and K. pneumoniae from Turkey using both phenotypic and genotypic methods and screening for the prevalence of gene coding for common aminoglycoside-modifying enzymes (AMEs) and 16S rRNA methylase genes. A total of 88 consecutive, non-duplicated E. coli (n = 65) and K. pneumoniae (n = 23) isolates showing resistance or intermediate resistance to amikacin and/or gentamicin were collected between October 2013 and May 2015 from clinical samples received at Gaziantep Dr. Ersin Arslan Training and Research Hospital. Seventeen isolates were obtained from Syrian patients. Isolates resistant to any of the two aminoglycosides were tested by PCR for seven AME genes, and 22 isolates with amikacin MIC ≥16 mg/L were also tested for 16S rRNA methylase genes. In E. coli isolates, the most frequent genes were aac(6')-Ib (50 strains; 76.9%) and aac(3)-IIa (40 strains; 70.7%), followed by aph(3')-Ia (5 strains; 7.6%) and ant(2″)-Ia (2 strains; 3.1%). Among the 23 resistant K. pneumoniae isolates, the most prevalent gene was aac(3')-IIa (87.0%) followed by aac(6')-Ib (73.9%) and aph(3')-Ia (8.6%). The rmtC gene was detected in one K. pneumoniae isolate. Resistance to aminoglycosides in clinical isolates of E. coli and K. pneumoniae from our center is predominantly caused by AAC(6')-Ib and AAC(3)-II enzymes, while the occurrence of 16S rRNA methylases is so far limited.en16S rRNA methyltransferaseAminoglycoside-modifying enzymeAminoglycosidesAnti-Bacterial AgentsDrug Resistance, BacterialEscherichia coliEscherichia coli InfectionsGenes, BacterialGenotypeGenotyping TechniquesHumansKlebsiella InfectionsKlebsiella pneumoniaeMicrobial Sensitivity TestsMolecular EpidemiologyPrevalenceSyriaTurkeyresearch article30803254open accessEpidemiología molecularGenes bacterianosGenotipoInfecciones por Escherichia coliInfecciones por KlebsiellaPruebas de sensibilidad microbiana10.1556/030.66.2019.0051588-2640http://real.mtak.hu/97084/1/030.66.2019.005.pdf