Losada Mendez, JorgePalomares, FranciscaGomez, FranciscaRamirez-Lopez, PedroRamos-Soriano, JavierJose Torres, MariaMayorga, CristobalinaRojo, Javier2025-01-072025-01-072021-11-111554-8929https://hdl.handle.net/10668/24541Covalent conjugation of allergens to toll-like receptor (TLR) agonists appears to be a powerful strategy for the development of safety compounds for allergen-specific immunomodulatory response toward tolerance in allergy. In this work, we have synthesized two family of ligands, an 8-oxoadenine derivative as a ligand for TLR7 and a pyrimido[5,4-b]indole as a ligand for TLR4, both conjugated with a T-cell peptide of Pru p 3 allergen, the lipid transfer protein (LTP) responsible for LTP-dependent food allergy. These conjugates interact with dendritic cells, inducing their specific maturation, T-cell proliferation, and cytokine production in peach allergic patients. Moreover, they increased the Treg-cell frequencies in these patients and could induce the IL-10 production. These outcomes were remarkable in the case of the TLR7 ligand conjugated with Pru p 3, opening the door for the potential application of these allergen-adjuvant systems in food allergy immunotherapy.enAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/Monophosphoryl-lipid-aDendritic cellsInnate immunityImmunotherapyRecognitionVaccinesIdentificationModulationActivationGsk2245035research article34761908open access10.1021/acschembio.1c007651554-8937https://pubs.acs.org/doi/pdf/10.1021/acschembio.1c00765726638500002