Vila-Farres, XavierParra-Millan, RaquelSanchez-Encinales, VivianaVarese, MonicaAyerbe-Algaba, RafaelBayo, NuriaGuardiola, SalvadorPachon-Ibañez, María EugeniaKotev, MartinGarcia, JesusTeixido, MeritxellVila, JordiPachon, JeronimoGiralt, ErnestSmani, Younes2023-01-252023-01-252017-10-31Vila-Farrés X, Parra-Millán R, Sánchez-Encinales V, Varese M, Ayerbe-Algaba R, Bayó N, et al. Combating virulence of Gram-negative bacilli by OmpA inhibition. Sci Rep. 2017 Oct 31;7(1):1-11.http://hdl.handle.net/10668/11756Preventing the adhesion of pathogens to host cells provides an innovative approach to tackling multidrug-resistant bacteria. In this regard, the identification of outer membrane protein A (OmpA) as a key bacterial virulence factor has been a major breakthrough. The use of virtual screening helped us to identify a cyclic hexapeptide AOA-2 that inhibits the adhesion of Acinetobacter baumannii, Pseudomonas aeruginosa and Escherichia coli to host cells and the formation of biofilm, thereby preventing the development of infection in vitro and in a murine sepsis peritoneal model. Inhibition of OmpA offers a strategy as monotherapy to address the urgent need for treatments for infections caused by Gram-negative bacilli.enAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/Drug discovery and developmentPathogensAcinetobacter InfectionsAcinetobacter baumanniiAlveolar Epithelial CellsAnimalsAnti-Bacterial AgentsBacterial AdhesionBacterial Outer Membrane ProteinsBiofilmsCell LineDNA HelicasesDisease Models, AnimalEscherichia coliEscherichia coli InfectionsFemaleHumansMiceMice, Inbred C57BLMultifunctional EnzymesPeptidesPseudomonas InfectionsPseudomonas aeruginosaRNA HelicasesSepsisVirulence Factorsresearch article29089624open accessCélulasInfeccionesBiopelículasAcinetobacter baumanniiBacteriasSepsisTerapéuticaTécnicas In VitroTamizaje masivoEscherichia coliPseudomonas aeruginosa10.1038/s41598-017-14972-y2045-2322PMC5666006https://www.nature.com/articles/s41598-017-14972-y.pdfhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5666006/pdf