Jabalera, YleniaSola-Leyva, AlbertoGaglio, Salvatore CalogeroCarrasco-Jiménez, María P.Iglesias, Guillermo R.Perduca, MassimilianoJimenez-Lopez, Concepcion2022-08-182022-08-182021-07-28Jabalera Y, Sola-Leyva A, Gaglio SC, Carrasco-Jiménez MP, Iglesias GR, Perduca M, et al. Enhanced Cytotoxic Effect of TAT-PLGA-Embedded DOXO Carried by Biomimetic Magnetic Nanoparticles upon Combination with Magnetic Hyperthermia and Photothermia. Pharmaceutics. 2021 Jul 28;13(8):1168http://hdl.handle.net/10668/3914The synergy between directed chemotherapy and thermal therapy (both magnetic hyperthermia and photothermia) mediated by a nanoassembly composed of functionalized biomimetic magnetic nanoparticles (BMNPs) with the chemotherapeutic drug doxorubicin (DOXO) covered by the polymer poly(lactic-co-glycolic acid) (PLGA), decorated with TAT peptide (here referred to as TAT-PLGA(DOXO-BMNPs)) is explored in the present study. The rationale behind this nanoassembly lies in an optimization of the nanoformulation DOXO-BMNPs, already demonstrated to be more efficient against tumor cells, both in vitro and in vivo, than systemic traditional therapies. By embedding DOXO-BMNPs into PLGA, which is further functionalized with the cell-penetrating TAT peptide, the resulting nanoassembly is able to mediate drug transport (using DOXO as a drug model) and behaves as a hyperthermic agent (induced by an alternating magnetic field (AMF) or by laser irradiation with a laser power density of 2 W/cm2). Our results obtained using the HepG2 cell line show that there is a synergy between chemotherapy and thermal therapy that results in a stronger cytotoxic effect when compared to that caused by the soluble DOXO. This is probably due to the enhanced DOXO release occurring upon the application of the thermal therapy, as well as the induced local temperature rise mediated by BMNPs in the nanoassembly following exposition to AMF or to near-infrared (NIR) laser irradiation. These results represent a proof of concept demonstrating that TAT-PLGA(DOXO-BMNPs) can be used to efficiently combine therapies against tumor cells, which is a step forward in the transition from systemic to local treatments.enAtribución 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/PhotothermiaHyperthermiaBMNPsPLGAMagnetic nanoparticlesTAT peptideDoxorubicinHipertermiaEnergía fotovoltaicaEnergía solarEnergía térmicaCopolímero de ácido poliláctico-ácido poliglicólicoMagnetismoNanopartículasPéptidosDoxorrubicinaMedical Subject Headings::Technology and Food and Beverages::Technology, Industry, and Agriculture::Manufactured Materials::Magnets::Magnetite NanoparticlesMedical Subject Headings::Phenomena and Processes::Biological Phenomena::Ecological and Environmental Phenomena::Meteorological Concepts::Weather::TemperatureMedical Subject Headings::Chemicals and Drugs::Biomedical and Dental Materials::Polymers::Polyesters::Polyglycolic AcidMedical Subject Headings::Technology and Food and Beverages::Technology, Industry, and Agriculture::Technology::Biotechnology::BiomimeticsMedical Subject Headings::Anatomy::Cells::Cells, Cultured::Tumor Cells, Cultured::Cell Line, Tumor::Hep G2 CellsMedical Subject Headings::Chemicals and Drugs::Polycyclic Compounds::Polycyclic Hydrocarbons, Aromatic::Naphthacenes::Anthracyclines::Daunorubicin::DoxorubicinMedical Subject Headings::Phenomena and Processes::Physical Phenomena::Magnetic Phenomena::Magnetic FieldsMedical Subject Headings::Phenomena and Processes::Physical Phenomena::Magnetic PhenomenaMedical Subject Headings::Chemicals and Drugs::Macromolecular Substances::PolymersMedical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::PeptidesMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Equipment and Supplies::Optical Devices::LasersEnhanced Cytotoxic Effect of TAT-PLGA-Embedded DOXO Carried by Biomimetic Magnetic Nanoparticles upon Combination with Magnetic Hyperthermia and Photothermiaresearch article34452129Acceso abierto10.3390/pharmaceutics130811681999-4923PMC8398382