Goikoetxea, M JD'Amelio C M,Martínez-Aranguren, RGamboa, PGarcia, B EGómez, FFernández J,Bartra, JParra, AAlvarado, M IAlonso, M IGonzález, ETerrados, SMoya, CBlanca, NFeo-Brito, FVillalba, MDíaz-Perales, ASanz, M L2025-01-072025-01-0720161018-9068https://hdl.handle.net/10668/27794Component-based diagnosis on multiplex platforms is widely used in food allergy but its clinical performance has not been evaluated in nut allergy. To assess the diagnostic performance of a commercial protein microarray in the determination of specific IgE (sIgE) in peanut, hazelnut, and walnut allergy. sIgE was measured in 36 peanut-allergic, 36 hazelnut-allergic, and 44 walnut-allergic patients by ISAC 112, and subsequently, sIgE against available components was determined by ImmunoCAP in patients with negative ISAC results. ImmunoCAP was also used to measure sIgE to Ara h 9, Cora 8, and Jug r 3 in a subgroup of lipid transfer protein (LTP)-sensitized nut-allergic patients (positive skin prick test to LTP-enriched extract). sIgE levels by ImmunoCAP were compared with ISAC ranges. Most peanut-, hazelnut-, and walnut-allergic patients were sensitized to the corresponding nut LTP (Ara h 9, 66.7%; Cor a 8, 80.5%; Jug r 3, 84% respectively). However, ISAC did not detect sIgE in 33.3% of peanut-allergic patients, 13.9% of hazelnut-allergic patients, or 13.6% of walnut-allergic patients. sIgE determination by ImmunoCAP detected sensitization to Ara h 9, Cor a 8, and Jug r 3 in, respectively, 61.5% of peanut-allergic patients, 60% of hazelnut-allergic patients, and 88.3% of walnut-allergic patients with negative ISAC results. In the subgroup of peach LTP-sensitized patients, Ara h 9 sIgE was detected in more cases by ImmunoCAP than by ISAC (94.4% vs 72.2%, P The diagnostic performance of ISAC was adequate for hazelnut and walnut allergy but not for peanut allergy. sIgE sensitivity against Ara h 9 in ISAC needs to be improved.enAdultAllergensBiomarkersCase-Control StudiesCorylusFemaleHumansImmunoglobulin EIntradermal TestsJuglansMaleMediterranean RegionMiddle AgedNut HypersensitivityNutsPeanut HypersensitivityPlant ProteinsPredictive Value of TestsProtein Array AnalysisSpainYoung Adultresearch article27012014