Palacín, ArantxaGómez-Casado, CristinaRivas, Luis AAguirre, JacoboTordesillas, LeticiaBartra, JoanBlanco, CarlosCarrillo, TeresaCuesta-Herranz, JavierFrutos, Consolación deGarcía Alvarez-Eire, GenovevaFernández, Francisco JGamboa, PedroMuñoz, RosaSánchez-Monge, RosaSirvent, SofíaTorres, María JVarela-Losada, SusanaRodríguez, RosalíaParro, VictorBlanca, MiguelSalcedo, GabrielDíaz-Perales, Araceli2013-07-102013-07-102012-12-14Palacín A, Gómez-Casado C, Rivas LA, Aguirre J, Tordesillas L, Bartra J, et al. Graph based study of allergen cross-reactivity of plant lipid transfer proteins (LTPs) using microarray in a multicenter study. PLoS ONE. 2012; 7(12):e50799http://hdl.handle.net/10668/1212The study of cross-reactivity in allergy is key to both understanding. the allergic response of many patients and providing them with a rational treatment In the present study, protein microarrays and a co-sensitization graph approach were used in conjunction with an allergen microarray immunoassay. This enabled us to include a wide number of proteins and a large number of patients, and to study sensitization profiles among members of the LTP family. Fourteen LTPs from the most frequent plant food-induced allergies in the geographical area studied were printed into a microarray specifically designed for this research. 212 patients with fruit allergy and 117 food-tolerant pollen allergic subjects were recruited from seven regions of Spain with different pollen profiles, and their sera were tested with allergen microarray. This approach has proven itself to be a good tool to study cross-reactivity between members of LTP family, and could become a useful strategy to analyze other families of allergens.enReacciones CruzadasEstudios multicéntricos como asuntoHipersensibilidadAnálisis de micromatricesInmunoanálisisEspañaMedical Subject Headings::Phenomena and Processes::Immune System Phenomena::Immune System Processes::Antigen-Antibody Reactions::Cross ReactionsMedical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Carrier ProteinsMedical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Plant ProteinsMedical Subject Headings::Chemicals and Drugs::Biological Factors::Antigens::Antigens, PlantMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Epidemiologic Methods::Epidemiologic Study Characteristics as Topic::Clinical Trials as Topic::Multicenter Studies as TopicMedical Subject Headings::Diseases::Immune System Diseases::HypersensitivityMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Microchip Analytical Procedures::Microarray AnalysisMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Immunologic Techniques::ImmunoassayMedical Subject Headings::Anatomy::Plant Structures::Plant Components, Aerial::FruitMedical Subject Headings::Organisms::Eukaryota::PlantsMedical Subject Headings::Geographicals::Geographic Locations::Europe::SpainMedical Subject Headings::Anatomy::Plant Structures::Plant Components, Aerial::Flowering Tops::Flowers::Germ Cells, Plant::Pollenresearch article23272072open access10.1371/journal.pone.00507991932-6203PMC3522694