Ruiz, PilarCausse, ManuelVaquero, ManuelGutierrez, Juan BautistaCasal, Manuel2025-01-072025-01-072017-12-061178-6973https://hdl.handle.net/10668/25740A new automated real-time PCR assay for the detection of rifampicin (RIF) and isoniazid (INH) resistance in Mycobacterium tuberculosis (MTB) was evaluated. A total of 163 clinical samples (128 pulmonary and 35 extra-pulmonary) were processed using four PCR assay kits: Abbott RealTime MTB RIF/INH, Genotype MTBDRplus, Xpert/MTB RIF, and Anyplex MTB/MDR. The results of phenotypic drug-susceptibility testing using BACTECMGIT 960 were used as reference. The sensitivity and specificity of the new Abbott RealTime MTB RIF/INH assay in comparison with phenotypic testing was 96.3% (95%CI 87.32%-100%) for RIF and 100% (95%CI 99.3%-100%) for INH; the sensitivity was 78.8% (95%CI 66.8%-90.9%) and the specificity was 100% (95%CI 98.9%-100%). The Abbott RealTime MTB RIF/INH test could be a valid method for detecting the most common mutations in strains resistant to RIF and INH.enAttribution-NonCommercial 4.0 Internationalhttp://creativecommons.org/licenses/by-nc/4.0/Abbott RealTime MTB RIF/INH Resistance assayM. tuberculosismutationsresearch article29263682open access10.2147/IDR.S147272PMC5724413https://www.dovepress.com/getfile.php?fileID=39599https://pmc.ncbi.nlm.nih.gov/articles/PMC5724413/pdf