Aguilar-Garcia, DanielFernandez-Sarmiento, J AndresDel Mar Granados Machuca, MariaRodriguez, Juan MorgazRascon, Pilar MuñozCalvo, Rocio NavarreteRuiz, Yolanda MillanPoveda, Jose Maria CarrilloCastañeda, Juan MuñozBertomeu, Ramon CugatDominguez Perez, Juan Manuel2023-05-032023-05-032022-10-17Aguilar-García D, Fernández-Sarmiento JA, Del Mar Granados Machuca M, Rodríguez JM, Rascón PM, Calvo RN, et al. Histological and biochemical evaluation of plasma rich in growth factors treatment for grade II muscle injuries in sheep. BMC Vet Res. 2022 Nov 12;18(1):400http://hdl.handle.net/10668/20311The purpose of this study was to perform a histological and biochemical evaluation of the influence of plasma rich in growth factors (PRGF) on muscle regeneration process after a surgically induced grade II muscle laceration. A randomized, single blind, controlled experimental research was conducted including twenty-one adult healthy sheep, randomly divided in three groups (n = 7). A grade II surgical section was performed in the biceps femoris muscle of both hindlimbs. After two days (basal time), intralesional infiltration of autologous PRGF or Saline solution was randomly administered in both hindlimbs. Treatment was repeated once a week. Animal groups were euthanized at 1 (T1), 2 (T2) or 4 (T4) weeks. Histological assessment showed that PRGF intralesional injection induced a significant decrease of inflammatory cells density, significant higher centrally nucleated fibers percentage and significantly smaller fibrotic areas compared to Saline-treated muscles at T1, T2 and T4. Also, lower vascular density, with lower capillaries cross-sectional area, in PRGF group compared to Saline was observed. Biochemical analysis revealed a significant higher expression level of MYOD1, MYF5 and MYOG genes in PRGF groups at T1 compared to Saline treated muscles. At ultrastructural level, PRGF groups presented scarce edema and loss of connective tissue structure, as well as higher mitochondrial density adequately associated to the sarcomere unit in contrast to the Saline group. In conclusion, histological, biochemical, and ultrastructural results showed that PRGF treatment improved muscle regeneration process leading to more mature histological aspect in newly formed muscle tissue after a surgically induced grade II muscle injury.enAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/Muscle injuryMuscle repairPlasma rich in growth factorsPlatelet-rich plasmaSheepAnimalsSingle-blind methodEuthanasia, animalIntercellular signaling peptides and proteinsPlasmaMusclesPlatelet-rich plasmaresearch article36371198open accessEutanasia animalMétodo simple ciegoMúsculosOvinosPlasmaPlasma rico en plaquetasPéptidos y proteínas de señalización intercelular10.1186/s12917-022-03491-21746-6148PMC9652891https://bmcvetres.biomedcentral.com/counter/pdf/10.1186/s12917-022-03491-2https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9652891/pdf