Testing of Diagnostic Methods for Detection of Influenza Virusfor Optimal Performance in the Context of an Influenza Surveillance Network

Pérez-Ruiz, MercedesYeste, RuthRuiz-Pérez, María-JoséRuiz-Bravo, Alfonsode la Rosa-Fraile, ManuelNavarro-Marí, José María2015-12-222015-12-222007-09Pérez-Ruiz M, , Yeste R, Ruiz-Pérez MJ, Ruiz-Bravo A, de la Rosa-Fraile M , Navarro-Marí JM. Testing of Diagnostic Methods for Detection of Influenza Virus for Optimal Performance in the Context of an Influenza Surveillance Network. J Clin Microbiol. 2007 Sep;45(9):3109-100095-1137http://hdl.handle.net/10668/2113Influenza surveillance networks must detect early the viruses that will cause the forthcoming annual epidemics and isolate the strains for further characterization. We obtained the highest sensitivity (95.4%) with a diagnostic tool that combined a shell-vial assay and reverse transcription-PCR on cell culture supernatants at 48 h, and indeed, recovered the strainenHumanosGripe humanaPatología molecularOrthomyxoviridaeSensibilidad y especificidadVigilancia de guardiaVirologíaCultivo de virusMedical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::HumansMedical Subject Headings::Diseases::Virus Diseases::RNA Virus Infections::Orthomyxoviridae Infections::Influenza, HumanMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Diagnosis::Diagnostic Techniques and Procedures::Clinical Laboratory Techniques::Molecular Diagnostic TechniquesMedical Subject Headings::Organisms::Viruses::RNA Viruses::OrthomyxoviridaeMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Epidemiologic Methods::Statistics as Topic::Sensitivity and SpecificityMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Epidemiologic Methods::Sentinel SurveillanceMedical Subject Headings::Disciplines and Occupations::Natural Science Disciplines::Biological Science Disciplines::Biology::Microbiology::VirologyMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Diagnosis::Diagnostic Techniques and Procedures::Clinical Laboratory Techniques::Microbiological Techniques::Virus CultivationTesting of Diagnostic Methods for Detection of Influenza Virusfor Optimal Performance in the Context of an Influenza Surveillance Networkresearch article17652478open access10.1128/JCM.00697-07.1098-660XPMC2045238