Nabi, DalilehDrechsler, HaukePschirer, JohannesKorn, FranzSchuler, NadineDiez, StefanJessberger, RolfChacón, Mariola2022-11-282022-11-282021-11-11Nabi D, Drechsler H, Pschirer J, Korn F, Schuler N, Diez S, et al. CENP-V is required for proper chromosome segregation through interaction with spindle microtubules in mouse oocytes. Nat Commun. 2021 Nov 11;12(1):6547http://hdl.handle.net/10668/4413Proper chromosome segregation is essential to avoid aneuploidy, yet this process fails with increasing age in mammalian oocytes. Here we report a role for the scarcely described protein CENP-V in oocyte spindle formation and chromosome segregation. We show that depending on the oocyte maturation state, CENP-V localizes to centromeres, to microtubule organizing centers, and to spindle microtubules. We find that Cenp-V-/- oocytes feature severe deficiencies, including metaphase I arrest, strongly reduced polar body extrusion, increased numbers of mis-aligned chromosomes and aneuploidy, multipolar spindles, unfocused spindle poles and loss of kinetochore spindle fibres. We also show that CENP-V protein binds, diffuses along, and bundles microtubules in vitro. The spindle assembly checkpoint arrests about half of metaphase I Cenp-V-/- oocytes from young adults only. This finding suggests checkpoint weakening in ageing oocytes, which mature despite carrying mis-aligned chromosomes. Thus, CENP-V is a microtubule bundling protein crucial to faithful oocyte meiosis, and Cenp-V-/- oocytes reveal age-dependent weakening of the spindle assembly checkpoint.enAtribución 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/Chromosome segregationMicrotubulesOocytesAneuploidyMetaphaseSpindle polesSegregación cromosómicaMicrotúbulosOocitosAneuploidiaMetafasePolos del huso mitóticoMedical Subject Headings::Organisms::Eukaryota::AnimalsMedical Subject Headings::Phenomena and Processes::Genetic Phenomena::Genetic Processes::Cell Division::Cell Nucleus Division::Chromosome SegregationMedical Subject Headings::Check Tags::FemaleMedical Subject Headings::Phenomena and Processes::Cell Physiological Phenomena::Cell Physiological Processes::Cell Cycle::Cell Cycle Checkpoints::M Phase Cell Cycle CheckpointsMedical Subject Headings::Phenomena and Processes::Cell Physiological Phenomena::Cell Physiological Processes::Cell Cycle::Cell Division::Cell Nucleus Division::MeiosisMedical Subject Headings::Phenomena and Processes::Cell Physiological Phenomena::Cell Physiological Processes::Cell Cycle::Cell Division::Cell Nucleus Division::MetaphaseMedical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Rodentia::Muridae::Murinae::MiceMedical Subject Headings::Anatomy::Cells::Cellular Structures::Intracellular Space::Cytoplasm::Cytoplasmic Structures::Cytoskeleton::Microtubule-Organizing CenterMedical Subject Headings::Anatomy::Cells::Cellular Structures::Intracellular Space::Cytoplasm::Cytoplasmic Structures::Cytoskeleton::MicrotubulesMedical Subject Headings::Anatomy::Cells::Germ Cells::Ovum::Oocytesresearch article34764261Acceso abierto10.1038/s41467-021-26826-32041-1723PMC8586017