Gomez-Gonzalez, EmilioBarriga-Rivera, AlejandroFernandez-Muñoz, BeatrizNavas-Garcia, Jose ManuelFernandez-Lizaranzu, IsabelMunoz-Gonzalez, Francisco JavierParrilla-Giraldez, RubenRequena-Lancharro, DesireeGil-Gamboa, PedroRosell-Valle, CristinaGomez-Gonzalez, CarmenMayorga-Buiza, Maria JoseMartin-Lopez, MariaMuñoz, OlgaGomez-Martin, Juan CarlosRelimpio-Lopez, Maria IsabelAceituno-Castro, JesusPerales-Esteve, Manuel APuppo-Moreno, AntonioGarcia-Cozar, Francisco JoseOlvera-Collantes, LuciaGomez-Diaz, Raquelde Los Santos-Trigo, SilviaHuguet-Carrasco, MonserratRey, ManuelGomez, EmiliaSanchez-Pernaute, RosarioPadillo-Ruiz, JavierMarquez-Rivas, Javier2023-05-032023-05-032022-01-28Gomez-Gonzalez E, Barriga-Rivera A, Fernandez-Muñoz B, Navas-Garcia JM, Fernandez-Lizaranzu I, Munoz-Gonzalez FJ, et al. Optical imaging spectroscopy for rapid, primary screening of SARS-CoV-2: a proof of concept. Sci Rep. 2022 Feb 18;12(1):2356http://hdl.handle.net/10668/19585Effective testing is essential to control the coronavirus disease 2019 (COVID-19) transmission. Here we report a-proof-of-concept study on hyperspectral image analysis in the visible and near-infrared range for primary screening at the point-of-care of SARS-CoV-2. We apply spectral feature descriptors, partial least square-discriminant analysis, and artificial intelligence to extract information from optical diffuse reflectance measurements from 5 µL fluid samples at pixel, droplet, and patient levels. We discern preparations of engineered lentiviral particles pseudotyped with the spike protein of the SARS-CoV-2 from those with the G protein of the vesicular stomatitis virus in saline solution and artificial saliva. We report a quantitative analysis of 72 samples of nasopharyngeal exudate in a range of SARS-CoV-2 viral loads, and a descriptive study of another 32 fresh human saliva samples. Sensitivity for classification of exudates was 100% with peak specificity of 87.5% for discernment from PCR-negative but symptomatic cases. Proposed technology is reagent-free, fast, and scalable, and could substantially reduce the number of molecular tests currently required for COVID-19 mass screening strategies even in resource-limited settings.enAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/Red Andaluza de Diseño y Traslación de Terapias AvanzadasExudates and transudatesHumansMass screeningPoint-of-care testingProof of concept studySARS-CoV-2SalivaSpectroscopy, near-infraredresearch article35181702open accessEspectroscopía infrarroja cortaExudados y transudadosPrueba de estudio conceptualPruebas en el punto de atenciónSalivaTamizaje masivo10.1038/s41598-022-06393-32045-2322PMC8857323https://www.nature.com/articles/s41598-022-06393-3.pdfhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8857323/pdf