D'Ambrosi, SilviaVisser, AllerdienAntunes-Ferreira, MafaldaPoutsma, AnkieGiannoukakos, StavrosSol, NikSabrkhany, SiamackBahce, IdrisKuijpers, Marijke J EOude Egbrink, Mirjam G AGriffioen, Arjan WBest, Myron GKoppers-Lalic, DanijelaOudejans, CeesWürdinger, Thomas2023-02-092023-02-092021-09-13D'Ambrosi S, Visser A, Antunes-Ferreira M, Poutsma A, Giannoukakos S, Sol N, et al. The Analysis of Platelet-Derived circRNA Repertoire as Potential Diagnostic Biomarker for Non-Small Cell Lung Cancer. Cancers (Basel). 2021 Sep 16;13(18):4644.2072-6694http://hdl.handle.net/10668/18559Tumor-educated Platelets (TEPs) have emerged as rich biosources of cancer-related RNA profiles in liquid biopsies applicable for cancer detection. Although human blood platelets have been found to be enriched in circular RNA (circRNA), no studies have investigated the potential of circRNA as platelet-derived biomarkers for cancer. In this proof-of-concept study, we examine whether the circRNA signature of blood platelets can be used as a liquid biopsy biomarker for the detection of non-small cell lung cancer (NSCLC). We analyzed the total RNA, extracted from the platelet samples collected from NSCLC patients and asymptomatic individuals, using RNA sequencing (RNA-Seq). Identification and quantification of known and novel circRNAs were performed using the accurate CircRNA finder suite (ACFS), followed by the differential transcript expression analysis using a modified version of our thromboSeq software. Out of 4732 detected circRNAs, we identified 411 circRNAs that are significantly (p-valueenAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/biomarkerscircular RNAliquid biopsynon-small cell lung cancerplateletsHumansRNARNA, CircularCarcinoma, Non-Small-Cell LungRNA-SeqReverse TranscriptionDown-RegulationNuclear Receptor Interacting Protein 1Blood PlateletsLung NeoplasmsGene Expression ProfilingSequence Analysis, RNASoftwarePolymerase Chain Reactionresearch article34572871open accessARNARN CircularAnálisis de secuencia de ARNCarcinoma de pulmón decélulas no pequeñasHumanosNeoplasias pulmonaresPerfilación de la expresión génica plaquetasProgramas informáticosProteína de interacción con receptores nucleares 1RNA-SeqReacción en cadena de la polimerasa10.3390/cancers13184644PMC8468408https://www.mdpi.com/2072-6694/13/18/4644/pdf?version=1631790779https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8468408/pdf